The following antibodies were used in ChIP and immunoblotting assays: anti-Brd2 (A302-583A, Bethyl Labs), anti-Brd3 (A302-368A, Bethyl Labs), anti-Brd4 (A301-985A, Bethyl Labs and ab128874, abcam), anti-H3K27ac (ab4729, abcam), anti-β-catenin (610154, BD Biosciences), anti-c-Myc (9402S, Cell Signalling Technology), anti-β-actin (A1978, Sigma-Aldrich) and anti-Hsp60 (sc-13966, Santa Cruz Biotechnology).
Anti c myc 9402s
Manufactured by Cell Signaling Technology
Anti-c-Myc (9402S) is a primary antibody used for the detection and analysis of c-Myc protein. c-Myc is a transcription factor that plays a crucial role in cell growth, proliferation, and metabolism. This antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of c-Myc in biological samples.
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2 protocols using anti c myc 9402s
1
Antibodies Used for ChIP and Immunoblotting
2
Quantitative Western Blot Analysis of Cellular Proteins
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Cells were processed and lysed as previously described.38 The tumoroid samples were processed according to published methods.39 Equal amounts of protein were loaded in each lane of a sodium dodecyl sulfate 4%–12% polyacrylamide gel. Western blot analysis was performed by the standard method using the following primary antibodies: anti‐SMAD4 (ab40759; Abcam; 1:1000), anti‐TCF7 (2203S; Cell Signaling Technology; 1:1000), anti‐c‐Myc (9402S; Cell Signaling Technology; 1:1000), anti‐Jagged‐1 (sc‐8303; Santa Cruz Biotechnology; 1:200), and anti‐β‐actin (ab49900; Abcam; 1:10 000). Western blot images were analyzed, and bands were quantified using ImageJ software (version 1.50b; National Institutes of Health; https://imagej.nih.gov/ij/ ).
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