Whole blood was collected from Lgr5+-GFP mice on LFD or HFD following a 3–4 hr fast into K2-EDTA collection tubes (Sarstedt AG & Co; Numbrect, Germany), and immediately centrifuged (1500 × g; 4°C, 15 min) to separate plasma from red blood cells. Plasma Insulin levels were measured by a rat/mouse ELISA (EMD Millipore, Inc) with rat insulin standards using a spectrophotometer (Biorad iMark platereader) following the manufacturer’s instructions. Plasma glucose was determined via the glucose oxidase method with an Analox GM7 analyzer (Analox Inst., USA Inc, Lunenberg, MA), as described (Einstein, et al. 2010 (link); Huffman, et al. 2016 (link); Muzumdar, et al. 2009 (link)).
K2 edta collection tubes
Manufactured by Sarstedt
Sourced in Germany
K2-EDTA collection tubes are used for the collection and transportation of blood samples. They contain the anticoagulant K2-EDTA, which prevents the blood from clotting during the collection and handling process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using k2 edta collection tubes
1
Measuring Plasma Glucose and Insulin in Mice
2
Plasma Biomarker Analysis Protocol
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Whole blood was collected following a 4 h fast into K2-EDTA collection tubes (Sarstedt AG & Co; Numbrect, Germany), and plasma was separated from red blood cells by centrifugation (1,500 × g, 4°C, 15 min). Plasma free fatty acids (Wako Diagnostics, Richmond, VA, USA) and triglycerides (Sigma, St. Louis, MO) were analyzed using standard calorimetric assays. Plasma insulin levels were measured by a rat/mouse ELISA (EMD Millipore, Inc.) with rat insulin standards using a spectrophotometer (Biorad iMark platereader) following the manufacturer's instructions (36 (link), 37 (link)). In addition, a Bio-Plex MAGPIX Multiplex Reader (Biorad Inc., Hercules, CA) was used to measure the following 23 inflammatory mediators simultaneously in plasma: Eotaxin, G-CSF, GM-CSF, IFN-γ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IP-10, KC, LIF, LIX, M-CSF, MCP-1, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α, and VEGF.
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