For the images exemplified in Fig. 2a , Supplementary Fig. 2a and 3e , a Leica SP5 confocal with a 63×/NA 1.3 glycerol objective was used. A white light laser (470–670 nm) was used at 650 nm for excitation of Alexa647, and a PMT was used for detection.
Na 1.3 glycerol objective
Manufactured by Leica camera
The 63×/NA 1.3 glycerol objective is a high-magnification, high-numerical aperture lens designed for use with microscopes. It provides a magnification of 63× and a numerical aperture of 1.3, allowing for the collection of a large amount of light and the observation of fine details. The objective is optimized for use with glycerol as the immersion medium.
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Lab products found in correlation
2 protocols using na 1.3 glycerol objective
1
Confocal Imaging of Alexa647 Samples
2
Confocal Imaging of Alexa647 Samples
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For the images exemplified in Fig. 2a , Supplementary Fig. 2a and 3e , a Leica SP5 confocal with a 63×/NA 1.3 glycerol objective was used. A white light laser (470–670 nm) was used at 650 nm for excitation of Alexa647, and a PMT was used for detection.
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