Fenoterol hydrobromide

Ascorbic acid, isoproterenol ( + )-bitartrate salt, fenoterol hydrobromide, forskolin, and BimI were purchased from Sigma-Aldrich. Gö6983 was purchased from Selleck Chem. BODIPY-FL-GTPγS was from Life Technologies. Polyethyleneimine (PEI) 25 kDa linear polymer was purchased from Polysciences. Streptavidin-coated magnetic beads (Dynabeads) were purchased from New England Biolabs (catalog number S1420S). Complementary DNA encoding human β₂-AR, human Gαq, and long splice variant of Gαs were obtained from Open Biosystems. Human V_1AR cDNA was purchased from DNASU Plasmid Repository. Purified Gαq (Mus musculus), Gαs long (Rattus norvegicus), and Gβ₁γ₂ heterodimer were purchased as purified lyophilized proteins from Kerafast^{55 (link)}.

Fenoterol hydrobromide and L-(−)-Norepinephrine-(+)-bitartrate were purchased from Sigma-Aldrich (St. Louis, MO, USA) (catalog No. F1016 and 489350). Forskolin was purchased from Cayman Chemical (Ann Arbor, MI, USA) (catalog No. 11018). N-Methyldopamine hydrochloride was purchased from Alfa Aesar (Tewksbury, MA, USA) (catalog No. J60306). Mephenesin was purchased from Ambeed (Chicago, IL, USA) (catalog No. A307218).