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Sterile filtered fetal bovine serum

Manufactured by Merck Group
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Sourced in Germany

Sterile-filtered fetal bovine serum is a common cell culture supplement. It is derived from the blood of bovine fetuses and undergoes a filtration process to remove particulates and microorganisms, ensuring a sterile product.

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Lab products found in correlation

D maltose, by Merck Group (3 mentions) D fructose, by Merck Group (3 mentions) L cysteine, by Merck Group (3 mentions) D cellobiose, by Merck Group (3 mentions) Trace mineral supplement, by Merck Group (2 mentions) Vitamin supplement, by Merck Group (2 mentions) Powerwave ht microplate spectrophotometer, by Agilent Technologies (2 mentions) D2438, by Merck Group (2 mentions) Soft sided vinyl chamber, by Coy Laboratory Products (2 mentions) Hepg2, by Merck Group (2 mentions) Mcf 7, by Merck Group (2 mentions) Rpmi 1640 medium, by Merck Group (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Hepg2, by American Type Culture Collection (2 mentions) Trace mineral supplement, by American Type Culture Collection (1 mentions) Trace vitamin supplement, by American Type Culture Collection (1 mentions) M9 salts, by Teknova (1 mentions) Vitamin k1 hemin solution, by BD (1 mentions) Direct zol rna miniprep plus, by Zymo Research (1 mentions) 0.1 mm silica beads, by Biospec (1 mentions)

Spelling variants of this product

Bovine serum (80 citations) Bovine fetal serum (14 citations) Sterile fetal bovine serum (2 citations) Low endotoxin sterile-filtered fetal bovine serum (FBS; (2 citations)

5 protocols using sterile filtered fetal bovine serum

1

Cell Culture of Common Cancer Cell Lines

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MCF-7 (HTB-22, oestrogen receptor-positive human breast adenocarcinoma cells), HepG2 (HB-8065, human hepatocellular carcinoma cells) and HT-29 (HTB-38, human colon adenocarcinoma cells) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The MCF-7 and HepG2 cells were maintained in RPMI-1640 medium (Sigma-Aldrich, Munich, Germany), supplemented with 10% sterile-filtered fetal bovine serum (FBS) (Sigma-Aldrich, Germany) and 1% antibiotic (l-glutamine-penicillin-streptomycin) (Sigma-Aldrich, Germany) solution. HT-29 cells were maintained in DMEM medium (Sigma-Aldrich, Germany), supplemented with 10% sterile-filtered fetal bovine serum (FBS) (Sigma-Aldrich, Germany) and 1% antibiotic (l-glutamine-penicillin-streptomycin) (Sigma-Aldrich, Germany) solution. Cells were grown at 37 °C in a humidified incubator containing 5% CO2.
Kntayya S.B., Ibrahim M.D., Mohd Ain N., Iori R., Ioannides C, & Abdull Razis A.F. (2018). Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells. Nutrients, 10(6), 718.
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2

Transcriptomic Analysis of B. thetaiotaomicron

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Cultures of B. thetaiotaomicron VPI-5482 Δtdk and Δtdk Δspt were diluted 1:100 from overnight cultures into 5 mL of BHI medium, supplemented with 1% Vitamin K1-hemin (Becton Dickinson) or minimal medium [1× M9 salts (Teknova), 1% sterile-filtered fetal bovine serum (Sigma-Aldrich), 1% vitamin K1-hemin solution (Becton Dickinson), 1% trace mineral supplement (ATCC), 1% trace vitamin supplement (ATCC), 1 g/L D-(+)-cellobiose (Sigma-Aldrich), 1 g/L D-(+)-maltose (Sigma-Aldrich), 1 g/L D-(+)-fructose (Sigma-Aldrich) and 0.5 g/L L-cysteine (Sigma-Aldrich)] and grown to exponential phase (OD600 ~0.4) in anaerobic conditions. Each culture was pelleted in the anaerobic chamber, supernatants were decanted, and pellets were resuspended in 500 μL Trizol. Trizol suspensions underwent a step of bead-beating using ~500 μL of 0.1 mm silica beads (BioSpec). RNA was extracted with the Direct-Zol RNA MiniPrep Plus (Zymo Research) according to manufacturer’s instructions.
Brown E.M., Ke X., Hitchcock D., Jeanfavre S., Avila-Pacheco J., Nakata T., Arthur T.D., Fornelos N., Heim C., Franzosa E.A., Watson N., Huttenhower C., Haiser H.J., Dillow G., Graham D.B., Finlay B.B., Kostic A.D., Porter J.A., Vlamakis H., Clish C.B, & Xavier R.J. (2019). Bacteroides-derived sphingolipids are critical for maintaining intestinal homeostasis and symbiosis. Cell host & microbe, 25(5), 668-680.e7.
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3

Anaerobic Growth of Ruminococcus gnavus

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We grew R. gnavus ATCC 29149 in BHI medium (37 g/L) containing: 5% sterile-filtered fetal bovine serum (Sigma-Aldrich), 1% vitamin K1-hemin solution (BD Biosciene), 1% trace mineral supplement (ATCC), 1% vitamin supplement (ATCC), 1 g/L D-(+)-cellobiose (Sigma-Aldrich), 1 g/L D-(+)-maltose (Sigma-Aldrich), 1 g/L D-(+)-fructose (Sigma-Aldrich) and 0.5 g/L L-cysteine (Sigma-Aldrich). Growth occurred under anaerobic conditions (atmosphere 5% H2, 20% CO2, 75% N2) in a soft-sided vinyl chamber (Coy Laboratory Products, Michigan, USA). We sterilized the media using a Corning filter unit (0.22 µm pore diameter). All metabolite standards (Sigma-Aldrich) were brought to 100 mM in DMSO (Sigma-Aldrich, D2438) prior to dilution for dose assays. Overnight bacterial cultures were diluted 100-fold in appropriate media and 40 µL were dispensed per well in 384-well plates (low evaporation lid, Costar 3680) containing metabolites or DMSO control. The plates were shaken to ensure homogeneity and bacterial growth was monitored anaerobically (absorbance at 600 nm) in a microplate reader (PowerWave HT Microplate Spectrophotometer, BioTek) for 24 hours at 37°C without shaking. Values recorded for DMSO controls and metabolite-treated triplicates were averaged.
Franzosa E.A., Sirota-Madi A., Avila-Pacheco J., Fornelos N., Haiser H.J., Reinker S., Vatanen T., Brantley Hall A., Mallick H., McIver L.J., Sauk J.S., Wilson R.G., Stevens B.W., Scott J.M., Pierce K., Deik A.A., Bullock K., Imhann F., Porter J., Zhernakova A., Fu J., Weersma R.K., Wijmenga C., Clish C.B., Vlamakis H., Huttenhower C, & Xavier R.J. (2018). Gut microbiome structure and metabolic activity in inflammatory bowel disease. Nature microbiology, 4(2), 293-305.
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4

Anaerobic Growth of Ruminococcus gnavus

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We grew R. gnavus ATCC 29149 in BHI medium (37 g/L) containing: 5% sterile-filtered fetal bovine serum (Sigma-Aldrich), 1% vitamin K1-hemin solution (BD Biosciene), 1% trace mineral supplement (ATCC), 1% vitamin supplement (ATCC), 1 g/L D-(+)-cellobiose (Sigma-Aldrich), 1 g/L D-(+)-maltose (Sigma-Aldrich), 1 g/L D-(+)-fructose (Sigma-Aldrich) and 0.5 g/L L-cysteine (Sigma-Aldrich). Growth occurred under anaerobic conditions (atmosphere 5% H2, 20% CO2, 75% N2) in a soft-sided vinyl chamber (Coy Laboratory Products, Michigan, USA). We sterilized the media using a Corning filter unit (0.22 μm pore diameter). All metabolite standards (Sigma-Aldrich) were brought to 100 mM in DMSO (Sigma-Aldrich, D2438) prior to dilution for dose assays. Overnight bacterial cultures were diluted 100-fold in appropriate media and 40 μL were dispensed per well in 384-well plates (low evaporation lid, Costar 3680) containing metabolites or DMSO control. The plates were shaken to ensure homogeneity and bacterial growth was monitored anaerobically (absorbance at 600 nm) in a microplate reader (PowerWave HT Microplate Spectrophotometer, BioTek) for 24 hours at 37°C without shaking. Values recorded for DMSO controls and metabolite-treated triplicates were averaged.
Franzosa E.A., Sirota-Madi A., Avila-Pacheco J., Fornelos N., Haiser H.J., Reinker S., Vatanen T., Brantley Hall A., Mallick H., McIver L.J., Sauk J.S., Wilson R.G., Stevens B.W., Scott J.M., Pierce K., Deik A.A., Bullock K., Imhann F., Porter J., Zhernakova A., Fu J., Weersma R.K., Wijmenga C., Clish C.B., Vlamakis H., Huttenhower C, & Xavier R.J. (2018). Gut microbiome structure and metabolic activity in inflammatory bowel disease. Nature microbiology, 4(2), 293-305.
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5

HepG2 and MCF-7 Cell Culture

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HepG2 (HB-8065, human hepatocellular carcinoma cells) and MCF-7 (HTB-22, oestrogen receptor-positive human breast adenocarcinoma cells), were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The HepG2 and MCF-7 cells were routinely maintained and incubated at 37 °C with 5% CO2 in RPMI-1640 medium (Sigma-Aldrich, Munich, Germany), supplemented with 10% sterile-filtered fetal bovine serum (FBS) (Sigma-Aldrich, Germany) and 1% antibiotic (penicillin-streptomycin) (Sigma-Aldrich, Germany).
Arumugam A., Ibrahim M.D., Kntayya S.B., Mohd Ain N., Iori R., Galletti S., Ioannides C, & Abdull Razis A.F. (2020). Induction of Apoptosis by Gluconasturtiin-Isothiocyanate (GNST-ITC) in Human Hepatocarcinoma HepG2 Cells and Human Breast Adenocarcinoma MCF-7 Cells. Molecules, 25(5), 1240.
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