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C57bl 6j 000664

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C57BL/6J (000664) is a laboratory mouse strain. It is a widely used inbred strain that serves as a reference strain for many research applications.

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Lab products found in correlation

Cd40 002928, by Jackson ImmunoResearch (2 mentions) Cx3cr1creer 020940, by Jackson ImmunoResearch (2 mentions) Rag mice, by Taconic Biosciences (1 mentions) Raw 264.7 cells, by American Type Culture Collection (1 mentions) Gm500, by Tecniplast (1 mentions) D12492, by Research Diets (1 mentions) Onetouch ultra, by LifeScan (1 mentions) Ot 1 003831, by Jackson ImmunoResearch (1 mentions) B6 cg ifnar1tm1.2ees j, by Jackson ImmunoResearch (1 mentions) Ai14 007914 mice, by Jackson ImmunoResearch (1 mentions) Littermate controls, by Jackson ImmunoResearch (1 mentions) D12492, by LabDiet (1 mentions) Sjl j, by Jackson ImmunoResearch (1 mentions)

28 protocols using c57bl 6j 000664

1

Orthotopic Tumor Transplantation in Mice

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All experiments were reviewed and overseen by the institutional animal use and care committee at Oregon Health and Science University (OHSU) in accordance with National Institutes of Health guidelines for the humane treatment of animals. C57BL/6J (000664) or NU/J (002019) mice from Jackson Laboratory were used for orthotopic transplant and xenograft experiments, respectively, at the ages denoted in the paper.
Bhattacharyya S., Oon C., Kothari A., Horton W., Link J., Sears R.C, & Sherman M.H. (2020). Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer. The Journal of Experimental Medicine, 217(8), e20191805.
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2

Murine Models for Immunological Studies

Cited 1 time
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C57BL/6J (000664), CD4-Cre+Tg (017336), Stat6−/−(005977), Tbx21−/−(004648), Thy1.1 (000406), Il10Rb−/− (005027), Il10−/− (002250), and Prdm1fl/fl(008100) mice were purchased from the Jackson Laboratory. Rag−/− mice were purchased from Taconic Farms. Stat4−/− mice were provided by M. Kaplan (Indiana University). Vav-Bcl2 Tg mice were provided by A. Nussenzweig. Stat1−/− and Stat3fl/fl mice were provided by D. Levy (36 (link), 37 (link)). Stat5fl/fl mice have been described previously (38 (link)), and Prdm1-EYFP (Jax 008828) was provided by S. Crotty (La Jolla Institute for Allergy and Immunology) and E. Meffre (Yale University). All floxed mice were bred with CD4-Cre+ Tg mice. All animal studies were performed according to the National Institutes of Health guidelines for the use and care of live animals and were approved by the Institutional Animal Care and Use Committee of the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). All in vitro experiments included two technical replicates per experiment and were performed independently two or three times, as indicated in the figure legends. All in vivo experiments were performed independently two times, with four to seven animals per group. Flow cytometry was individually performed on each animal to provide statistics.
Poholek A.C., Jankovic D., Villarino A.V., Petermann F., Hettinga A., Shouval D.S., Snapper S.B., Kaech S.M., Brooks S.R., Vahedi G., Sher A., Kanno Y, & O’Shea J.J. (2016). IL-10 induces a STAT3-dependent autoregulatory loop in TH2 cells that promotes Blimp-1 restriction of cell expansion via antagonism of STAT5 target genes. Science immunology, 1(5), eaaf8612.
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3

Isolation and Culture of Mouse Immune Cells

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RAW 264.7 cells were purchased from ATCC. SRDC cells were a kind gift from Daniel Bout (University of Tours, France). Cells were maintained as previously described (Ruiz et al., 2005 (link)). Six- to eight-week old CD44 knockout (005085), CD36 knockout (019006) and matched control mice (C57BL/6J, 000664) were purchased from Jackson Laboratories. Bone marrow-derived macrophages (BMM) and dendritic cells (BMDC) were isolated as previously described (Wobus et al., 2004 (link)). All animal procedures were performed in compliance with University of Michigan and federal guidelines and the standards of the NIH Guide for the Care and Use of Laboratory Animals (2011) .
Cunha J.B, & Wobus C.E. (2016). Select membrane proteins modulate MNV-1 infection of macrophages and dendritic cells in a cell type-specific manner. Virus research, 222, 64-70.
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4

Mice Behavioral Testing Protocol

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All animal procedures were approved by the Institutional Animal Care and Use Committee at the Uniformed Services University of the Health Sciences (Bethesda, MD). Male and female mice, 8 weeks old, were obtained from Jackson Laboratories (C57BL/6J, 000664; Bar Harbor, Maine) and group-housed in Association for Assessment and Accreditation of Laboratory Animal Care-Accredited facilities with a standard 12-h light-dark cycle, with food and water available ad libitum. Animals acclimated to facilities for at least 1 week prior to baseline behavioral testing.
Tucker L.B., Velosky A.G., Fu A.H, & McCabe J.T. (2019). Chronic Neurobehavioral Sex Differences in a Murine Model of Repetitive Concussive Brain Injury. Frontiers in Neurology, 10, 509.
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5

Genetic Knockout Mice Model

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NdpKO mice (#012287) and WT mice (C57BL/6J #000664) were purchased from the Jackson Laboratory. Mice were housed and bred in a normal experimental room and exposed to a 12-hour light/dark cycle with free access to food and water. All procedures were performed in accordance with approved IACUC protocols at Stanford University (#12501) and Surrozen, Inc (#SRZ-IACUC-002).
Ding J., Lee S.J., Vlahos L., Yuki K., Rada C.C., van Unen V., Vuppalapaty M., Chen H., Sura A., McCormick A.K., Tomaske M., Alwahabi S., Nguyen H., Nowatzke W., Kim L., Kelly L., Vollrath D., Califano A., Yeh W.C., Li Y, & Kuo C.J. (2023). Therapeutic blood-brain barrier modulation and stroke treatment by a bioengineered FZD4-selective WNT surrogate in mice. Nature Communications, 14, 2947.
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6

Mouse Model for Breast Cancer

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Male and female C57Bl/6J (000664) and FVB/NJ (001800), or FVB/N MMTV-PyMT (FVB/N-Tg(MMTV-PyVT)634Mul/J; 002374) female mice were obtained from Jackson Laboratories; the mouse weight range, 24–30 g. All animal work was performed in accordance with the West Virginia University Animal Care and Use Committee (WVU IACUC) approved protocol.
Velayutham M., Poncelet M., Eubank T.D., Driesschaert B, & Khramtsov V.V. (2021). Biological Applications of Electron Paramagnetic Resonance Viscometry Using a 13C-Labeled Trityl Spin Probe. Molecules, 26(9), 2781.
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7

Mouse Behavioral Testing Protocols

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All animal procedures were approved by the Institutional Animal Care and Use Committee at the Uniformed Services University of the Health Sciences (USUHS; Bethesda, MD). Male and female mice, 8 weeks old, were obtained from Jackson Laboratories (C57BL/6J, 000664; Bar Harbor, Maine) and group-housed (4-5 per cage) in Association for Assessment and Accreditation of Laboratory Animal Care–accredited facilities with a standard 12-h light–dark cycle, with food (Harlan Teklad Global Diets 2018, 18% protein) and water available ad libitum. Animals acclimated to facilities for 7-10 days prior to baseline behavioral testing. All procedures involving rodent handling were performed by female investigators.44 (link)
Tucker L.B., Fu A.H, & McCabe J.T. (2021). Hippocampal-Dependent Cognitive Dysfunction following Repeated Diffuse Rotational Brain Injury in Male and Female Mice. Journal of Neurotrauma, 38(11), 1585-1606.
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8

Bdnf Knockout Mice for Behavioral Study

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Female adult mice (16–18 weeks old at the beginning of the experiments) of C57BL/6J-000664 background (from Jackson Laboratories, Bar Harbor, ME, USA, maintained in the Laboratory Animal Center of the University of Helsinki), carrying a deletion in one of the copies of Bdnf gene or wild-type littermates were used [17 (link)]. The animals were group housed (4–5/cage; type-II individually ventilated cages GM500, 391 × 199 × 160 mm, floor area 501 cm2; Tecniplast, Buguggiate, Varese, Italy) in a 12-h light/12-h dark cycle (light on at 7:00 a.m.), with free access to food and water except during the experimental sessions. All protocols were approved by the ethics committee for animal experimentation of Southern Finland (ESAVI/38503/2019).
Laukkanen L., Diniz C.R., Foulquier S., Prickaerts J., Castrén E, & Casarotto P.C. (2021). Facilitation of TRKB Activation by the Angiotensin II Receptor Type-2 (AT2R) Agonist C21. Pharmaceuticals, 14(8), 773.
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9

Genetic Engineering in Mouse Models

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All animals were housed in the animal maintenance facility at the University of Michigan Health System. This research was undertaken with the approval of the Committee on Use and Care of Animals at the University of Michigan under protocol #PRO00009914. Mouse genotypes were confirmed by PCR using mouse tails. Mouse strains (1) S100a8Cre #021614 (2), Cxcr2flox/flox #24638, and (3) C57BL/6J #000664 were all obtained from Jackson Labs (Bar Harbor, ME). S100a8CreCxcr2flox/flox were generated by crossing S100a8Cre mice to Cxcr2flox/flox mice. Mice were housed under specific pathogen-free conditions and fasted overnight before use, with free access to water.
Kao K.D., Grasberger H, & El-Zaatari M. (2023). The Cxcr2+ subset of the S100a8+ gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathology. Frontiers in Immunology, 14, 1147695.
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10

Bleomycin-Induced Lung Injury and MSC Therapy

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All animal experiments were performed in compliance with NIH and institutional guidelines approved by the ethical committee from the University of Illinois at Chicago (ACC number: 18–231). C57BL/6 J (000664) and B6;129S-Tnftm1Gkl/J (003008, Tnfα−/−) mice were purchased from the Jackson Laboratory. Mice (8 to 12 weeks old) were anesthetized with ketamine/xylazine (50/5 mg per kg), followed by single dose i.t. administration of bleomycin (0.015U per 20 g mouse) as described previously65 . Bleomycin was diluted in 30 μl PBS and instilled using a 100 μl pipette tip after exposing the laryngopharynx area of the anesthetized mice. Both nostrils were temporarily closed to facilitate liquid flow into the trachea. After 1 or 3 weeks of bleomycin administration, animals received a vehicle (sterile serum-free Fluorobrite DMEM), 100,000 uncoated MSCs, or 100,000 gel-coated MSCs in 30 μl Fluorobrite DMEM per 20 g mouse via the i.t. route as done with bleomycin, or through the i.v. route via retro-orbital injection with a 27 G syringe needle. After 1 week of cell delivery, animals were killed for downstream analyses.
Wan Wong S., Tamatam C.R., Cho I.S., Toth P.T., Bargi R., Belvitch P., Lee J.C., Rehman J., Reddy S.P, & Shin J.W. (2021). Inhibition of aberrant tissue remodelling by mesenchymal stromal cells singly coated with soft gels presenting defined chemomechanical cues. Nature biomedical engineering, 6(1), 54-66.
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