Ek0411

Manufactured by Boster Bio

Sourced in China

The EK0411 is a lab equipment product manufactured by Boster Bio. It is designed for general laboratory use, but its core function is not provided in a detailed, unbiased, and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Ek0527, by Boster Bio (7 mentions) Ek0394, by Boster Bio (5 mentions) Ek0568, by Boster Bio (1 mentions) Ek0513, by Boster Bio (1 mentions) Ek0460, by Boster Bio (1 mentions) Ek0466, by Boster Bio (1 mentions)

11 protocols using ek0411

Measuring Serum Cytokines in Mice

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Mouse blood samples were collected retro‐orbitally and centrifuged at 860 g for 20 min. The upper layer of serum was assayed using enzyme‐linked immunosorbent assay kits to measure the levels of IL‐1β (Bosterbio; #EK0394), IL‐6 (Bosterbio; #EK0411) and TNF‐α (Bosterbio; #EK0527).

Yang Y., Liu Y., He X., Yang F., Han S., Qin A., Wu G., Liu M., Li Z., Wang J., Yang X, & Hu D. (2020). ING4 alleviated lipopolysaccharide‐induced inflammation by regulating the NF‐κB pathway via a direct interaction with SIRT1. Immunology and Cell Biology, 98(2), 127-137.

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Hippocampal Cytokine Measurement Protocol

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The mouse hippocampus was collected, and the protein extraction was performed as in the previous method. IL-6 (EK0411, Boster Biological Technology Co. Ltd), IL-1β (EK0394, Boster Biological Technology Co. Ltd), and tumor necrosis factor (TNF)-α (EK0527, Boster Biological Technology Co. Ltd) ELISA kits were used to measure the levels of IL-6, IL-1β, and TNF-α in the mouse hippocampus tissue following the instructions from the manufacturer.

Sun J., Wu J., Hua F., Chen Y., Zhan F, & Xu G. (2020). Sleep Deprivation Induces Cognitive Impairment by Increasing Blood-Brain Barrier Permeability via CD44. Frontiers in Neurology, 11, 563916.

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Colonic Cytokine Profiling in Mice

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Colon inflammatory cytokine was assessed by flushing mice colons with PBS, and the distal-most 1-cm² colon sections were cultured in RPMI media containing penicillin/streptomycin for 24 h. Supernatants from these cultures were removed, cleared of debris by centrifugation and assessed for mouse IL-6 (EK0411), TNF-α (EK0527), IL-1β (EK0394) (Boster Biological Technology) by ELISA.

Du Q., Wang Y., Liu C., Wang H., Fan H., Li Y., Wang J., Zhang X., Lu J., Ji H, & Hu R. (2016). Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-β-catenin axis. Oncotarget, 7(14), 17870-17884.

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Cytokine Profiling in DDO7232 and DSS Treatment

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NCM460 cells were plated at a density of 7 × 10⁵ cells per 60 mm dish overnight. The cells were treated with a density gradient of DDO7232 and/or 40 mg/kg DSS for 24 h. The cell supernatant was assayed for IL-6 (EK0411, ELISA kit, Boster, China), IL-1β (EK0393, ELISA kit, Boster, China), and TNF-α (EK0527, ELISA kit, Boster, China) using double-sandwich ELISA techniques, as previously described.

Xu L.L., Liu T., Wang L., Li L., Wu Y.F., Li C.C., Di B., You Q.D, & Jiang Z.Y. (2018). 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation. Oxidative Medicine and Cellular Longevity, 2018, 3271617.

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Quantifying Cytokine Profiles in mTECs

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Mouse TGF-β1, IL-6, TNF-α, and CCL2 levels in the supernatants of mTECs were measured using enzyme-linked immunosorbent assay (ELISA) kits (EK0515, EK0411, EK0527, EK0568, BOSTER, China) in accordance with manufacturer’s instructions.

Gong W., Luo C., Peng F., Xiao J., Zeng Y., Yin B., Chen X., Li S., He X., Liu Y., Cao H., Xu J, & Long H. (2021). Brahma-related gene-1 promotes tubular senescence and renal fibrosis through Wnt/β-catenin/autophagy axis. Clinical Science (London, England : 1979), 135(15), 1873-1895.

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Measuring Mouse Cytokine Levels

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Mouse blood samples were collected retro-orbitally and centrifuged at 3,000 rpm for 20 min. The upper layer of the serum was then assayed using ELISA kits to measure the levels of interleukin (IL)-1β (Bosterbio, #EK0394) and IL-6 (Bosterbio, #EK0411).

Yang Y., Liu M., Yang F., Wang X., Bai X., Mu S., Liu Y, & Hu D. (2022). Circular RNA expression profiles following negative pressure wound therapy in burn wounds with experimental Pseudomonas aeruginosa infection. Bioengineered, 13(2), 4122-4136.

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Evaluating ECM Proteins and TGF-β1

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CF culture media was collected to detect Col-I/III and TGF-β₁ levels using ELISA detection kits (cat. nos. EK0411, EK0424 and EK0513, respectively; Boster Biological Technology) according to the manufacturer's protocols.

Yuan H., Xu J., Zhu Y., Li L., Wang Q., Yu Y., Zhou B., Liu Y., Xu X, & Wang Z. (2020). Activation of calcium-sensing receptor-mediated autophagy in high glucose-induced cardiac fibrosis in vitro. Molecular Medicine Reports, 22(3), 2021-2031.

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Quantifying Inflammatory Cytokines in LPS-Stimulated Cells

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After overnight culture in a 24-well plate (1 × 10⁵ cells/well, 500 μL medium/well), the cells were pre-treated with punicalagin for 1 h and lipopolysaccharide for an additional 24 h, the culture supernatant from each well was collected at the end of scheduled experiments and used to measure TNF-α (EK0527, BOSTER, Wuhan, China) and IL-6 (EK0411, BOSTER, Wuhan, China) concentration by ELISA according to the manufacturer’s instructions, and the absorbance was then measured at 520 nm using a microplate reader.

Cao Y., Chen J., Ren G., Zhang Y., Tan X, & Yang L. (2019). Punicalagin Prevents Inflammation in LPS- Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway. Nutrients, 11(11), 2794.

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Measuring Cytokine Levels in Serum

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Blood samples were centrifuged at 1000 g for 10 min. To detect concentration of IL-6 (EK0411; Boster, USA) and TNFα (1217202; Dekewe, China) in serum, serum was used with enzyme-linked immunosorbent assay.

Tang L., Mei X., Ye M., Liu Y., Huang Y., Yu J., Zhang L., Zhuge S., Jiang G, & Zhu J. (2023). Sclareol ameliorates liver injury by inhibiting nuclear factor-kappa B/NOD-like receptor protein 3-mediated inflammation and lipid metabolism disorder in diabetic mice. International Journal of Immunopathology and Pharmacology, 37, 03946320231223644.

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Quantification of Alzheimer's Biomarkers

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Animals were sacrificed, the brains were quickly harvested, and the hippocampus homogenized in liquid nitrogen. Concentrations of Aβ40, Aβ42 (R&D Systems, DAB140B, DAB142), HbA1c (MSK, kt20296), IL-6, IL-1β, INF-γ, and TNF-α (Boster, EK0411, EK0394, EK0375, and EK0527, respectively) in tissue homogenate were quantitatively measured by ELISA according to manufacturers’ instructions.

He C., Li Q., Cui Y., Gao P., Shu W., Zhou Q., Wang L., Li L., Lu Z., Zhao Y., Ma H., Chen X., Jia H., Zheng H., Yang G., Liu D., Tepel M, & Zhu Z. (2022). Recurrent moderate hypoglycemia accelerates the progression of Alzheimer’s disease through impairment of the TRPC6/GLUT3 pathway. JCI Insight, 7(5), e154595.

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