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Model 1100

Manufactured by PerkinElmer
Sourced in United States

The Model 1100 is a laboratory equipment designed for general analytical applications. It features a compact design and provides essential functionalities for various laboratory tasks.

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6 protocols using model 1100

1

Arsenic Adsorption by FeOOH Nanoparticles

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The desired amount (1–8 g L−1) of FeOOH, which consists of nanosized akaganeite, goethite, and lepidocrocite [23 (link)] was added to Erlenmeyer flasks with a 50 mL solution of arsenate with an arsenic concentration of 100 mg L−1. The stock solution of arsenate was prepared from Na2HAsO4.7H2O (ACS reagent, ≥98%; Sigma–Aldrich, Taufkirchen, Germany), which was dissolved using redistilled water. A FeOOH-free run was also tested.
The prepared suspensions were placed onto a horizontal shaker (Unimax 2010; Heidolph, Schwabach, Germany), and agitated at 130 rpm for 24 h in the dark at 25 °C. Thereafter, the suspensions were filtered through a 0.45 µm pore-size mixed cellulose esters (MCE) membrane filter. The filtrates were diluted to 100 mL with redistilled water and there the total content of arsenic was determined by flame atomic absorption spectrometry (F-AAS; Perkin Elmer Model 1100 (Waltham, USA), wavelength 193.7 nm, air-acetylene flame, deuterium background correction, LOQ ~ 0.5 mg L−1, the standard expanded uncertainty (k = 2) is 6%) or inductively coupled plasma mass spectrometry (ICP-MS; Thermo Scientific (Waltham, USA) iCap Q in KED (kinetic energy discrimination) mode (He), LOQ ~ 0.005 mg L−1, the standard expanded uncertainty (k = 2) is 4%).
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2

Lentil Seed Mineral Analysis

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The final lentil seed mineral concentration was analyzed after digestion of 0.15–0.30 g seed samples. This was performed by the Anton Paar Multiwave 3000 microwave digestion system (Graz, Austria) in PTFE pressure vessels using the concentrated HNO3 and H2O2 mixture at 60 bar pressure. Iron, zinc, magnesium, calcium and phosphor content was determined by ICP-OES (Varian Vista MPX, Mulgrave, Victoria, Australia) with yttrium as the internal standard. Potassium, copper and manganese were then provided by F-AAS (Perkin-Elmer Model 1100, Waltham, MA, USA) [41 ]. Finally, the Kjeldahl method determined total nitrogen, and colorimetry established sulfur content.
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3

Powder FTIR Spectroscopic Analysis

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FTIR spectra were recorded using a Perkin Elmer Model 1100 at room temperature. The wavelength range was from 4000 to 500 cm−1. Each powder sample was sandwiched between two KBr pellets. The spectra were obtained using 32 scans at a resolution of 4 cm−1.
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4

Bioextraction of Arsenic from FeOOH

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To determine bioextraction efficiency of arsenic from the FeOOH phase collected after the sorption experiment, a one-step extraction using A. niger strain was applied. The recovered FeOOH on the membrane filter was placed in a 100 mL Erlenmeyer flask, and sterilized in the laboratory oven at 70 °C for 10 h. The day prior to inoculation with A. niger spore suspension, each sample had been sterilized for another 40 min at 95 °C, and 50 mL of sterilized Sabouraud dextrose broth medium was added. After inoculation, the fungus was cultivated for 19 days in the dark at 25 °C in the laboratory incubator. After a 19-day cultivation period, the fungal biomass was collected, rinsed with redistilled water, and air-dried to constant weight. The spent growth medium was filtered through a 0.45 µm pore-size MCE membrane filter to separate the remaining FeOOH phase from the growth medium. The collected FeOOH phases were digested in a microwave system with a mixture of concentrated acids (HF + HNO3 + HClO4 + H2O2). This was followed by determination of total arsenic content using ICP–MS or F–AAS [25 ]. Iron was determined by flame atomic absorption spectrometry (F–AAS, Perkin Elmer Model 1100 (Waltham, USA), wavelength 248.3 nm, air-acetylene flame, deuterium background correction, LOQ ~ 0.02 mg L−1, the standard expanded uncertainty (k = 2) is 4%).
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5

Cardiovascular and Respiratory Responses

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Heart rate (HR) and rhythm were monitored continuously using a 12-lead electrocardiogram. Blood pressure was monitored via arterial waveform tracings. Gas exchange, including ventilation ( V E ), oxygen uptake ( VO 2 ), and carbon dioxide elimination ( VCO 2 ), was measured using a customized breath-by-breath measurement system (Beck Integrative Physiological System, BIPS; KCBeck, Physiological Consulting, Liberty, UT, USA) integrated with a mass spectrometer (Perkin-Elmer, model 1100). These measurements were made at rest, during the final minute of constant-load cycling, and at peak exercise (data not shown).
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6

Standardized Exercise Testing Protocol

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Exercise testing was conducted in accordance with the American College of Sports Medicine guidelines (19 ). Electrocardiograms and pulse oximetry were monitored continuously during the test. Heart rate and blood pressure were recorded during the final 30 seconds of each stage. Expired air was analyzed breath-by-breath for O2 and CO2 using either a mass spectrometer (Model 1100; Perkin-Elmer Corp., Waltham, MA; n = 7) or an automated metabolic cart (Cardio2; MGC, St. Paul, MN; n = 17). The following variables were calculated and averaged sequentially every 15 seconds during the exercise: expired ventilation
(V˙E) , oxygen consumption
(V˙O2) , carbon dioxide production
(V˙CO2) , heart rate, and
SaO2 . All instruments were calibrated before each test.
Subjects walked on a motorized treadmill at a comfortable pace (range, 1.3–3.5 mph) on the level during a 2- to 4-minute warm-up phase. Subsequently, treadmill speed remained constant while grade increased 2% every 2 minutes until the subject requested that the test be stopped (symptom limited) or test termination criteria were met. Tests were terminated for: (1 (link)) heart rate greater than or equal to 90% of age-predicted maximum, (2 (link)) a
V˙O2 of 25 ml/kg/min, or (3 (link)) any of the standard clinical safety end points.
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