Calcium chloride dihydrate

Manufactured by Scharlab

Sourced in Spain, Australia

Calcium chloride dihydrate is an inorganic compound with the chemical formula CaCl₂·2H₂O. It is a crystalline solid that is highly soluble in water. Calcium chloride dihydrate is commonly used as a desiccant, a freezing point depressant, and in various industrial processes.

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6 protocols using calcium chloride dihydrate

Preparation and Characterization of Ion Exchange Membranes

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Sodium hypochlorite (NaClO, 14%), sodium hydrogen phosphate (NaH₂PO₄), sodium chloride (NaCl), calcium chloride dihydrate (CaCl₂∙2H₂O), magnesium sulfate heptahydrate (MgSO₄∙7H₂O), sodium hydrogen carbonate (NaHCO₃), potassium nitrate (KNO₃), sodium nitrate (NaNO₃), calcium carbonate (CaCO₃), magnesium chloride hexahydrate (MgCl₂∙6H₂O), potassium sulfate (K₂SO₄), sodium sulfate (Na₂SO₄), calcium sulfate dihydrate (CaSO₄∙2H₂O), boric acid (H₃BO₃, 0.5%), hydrogen peroxide (H₂O₂, 30%), and chloride acid (HCl, 0.1 M) were purchased from Scharlab S.L., Barcelona, Spain. The ultrapure water (Milli-Q) used in the experiments was obtained from Millipore, Molsheim, France, equipment (conductivity less than 0.055 µS cm⁻¹).
Polyvinyl chloride (PVC, Mw 112,000 g mol⁻¹) was supplied by ATOCHEM, Madrid, Spain. Tetrahydrofuran (THF) was purchased from Scharlab S.L. Amberlite^® IRA-402 (Cl⁻ form, total exchange capacity ≥ 1.0 mol L⁻¹) was supplied by Merck Life Science, Darmstadt, Germany, S.L.U.

Pompa-Pernía A., Molina S., Lejarazu-Larrañaga A., Landaburu-Aguirre J, & García-Calvo E. (2022). Validation of Recycled Nanofiltration and Anion-Exchange Membranes for the Treatment of Urban Wastewater for Crop Irrigation. Membranes, 12(8), 746.

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Synthesis of Polymer Composites

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PB (98%), low-viscosity SA (99%), and DCA (98%) were purchased from Sigma-Aldrich Co., (St Louis, MO, USA). Calcium chloride dihydrate (CaCl₂·2H₂O, 98%) was obtained from Scharlab S.L (Sentmenat, Spain). All solvents and reagents were supplied by Merck KGaA (Darmstadt, Germany) and were of high-performance liquid chromatography grade and used without further purification.

Mooranian A., Negrulj R., Chen-Tan N., Watts G.F., Arfuso F, & Al-Salami H. (2014). An optimized probucol microencapsulated formulation integrating a secondary bile acid (deoxycholic acid) as a permeation enhancer. Drug Design, Development and Therapy, 8, 1673-1683.

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Metformin-Alginate Nanoparticle Formulation

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Metformin (M, 98%), sodium alginate (SA, 99%), chenodeoxycholic acid (CDCA, 99%) and Poloxamer 407 were purchased from Sigma Chemical Co (St. Louis, MO, USA). Eudragit^® RS30D, RL30D and NM30D polymers were supplied by Evonik Industries (Essen, Germany). Calcium chloride dihydrate (CaCl₂ 2H₂O 98%) was obtained from Scharlab S.L (Barcelona, Spain). All reagents and solvents were HPLC grade, used without further purification or modification and were supplied by Merck (Bayswater, VIC, Australia).

Mooranian A., Carey L., Ionescu C.M., Walker D., Jones M., Wagle S.R., Kovacevic B., Foster T., Chester J., Johnston E., Mikov M, & Al-Salami H. (2021). The Effects of Accelerated Temperature-Controlled Stability Systems on the Release Profile of Primary Bile Acid-Based Delivery Microcapsules. Pharmaceutics, 13(10), 1667.

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Reagent Procurement for Chemical Analysis

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G (99.92%), LVSA (99%), and DCA (99%) were purchased from Sigma-Aldrich Co (St Louis, MO, USA). Calcium chloride dihydrate ([CaCl₂ · 2H₂O] 98%) was obtained from Scharlab SL (Barcelona, Spain). All solvents and reagents were supplied by Merck (Darmstadt, Germany), and were of high-performance liquid chromatography (HPLC) grade and used without further purification.

Mooranian A., Negrulj R., Chen-Tan N., Al-Sallami H.S., Fang Z., Mukkur T., Mikov M., Golocorbin-Kon S., Fakhoury M., Arfuso F, & Al-Salami H. (2014). Novel artificial cell microencapsulation of a complex gliclazide-deoxycholic bile acid formulation: a characterization study. Drug Design, Development and Therapy, 8, 1003-1012.

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Composite Alginate-Based Metformin Formulation

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Metformin (M, 98%), sodium alginate (SA, 99%) and chenodeoxycholic acid (CDCA, 99%) purchased from Sigma Chemical Co, USA. Calcium chloride dihydrate (CaCl₂.2H₂O 98%) was obtained from Scharlab S.L, Australia. All reagents and solvents were HPLC grade used without further purification or modification and were supplied by Merck (Australia).

Mooranian A., Foster T., Ionescu C.M., Carey L., Walker D., Jones M., Wagle S.R., Kovacevic B., Chester J., Johnstone E., Kuthubutheen J., Brown D., Atlas M.D., Mikov M, & Al-Salami H. (2021). The Effects of Primary Unconjugated Bile Acids on Nanoencapsulated Pharmaceutical Formulation of Hydrophilic Drugs: Pharmacological Implications. Drug Design, Development and Therapy, 15, 4423-4434.

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Probucol-Alginate-Cholic Acid Formulation

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Probucol (PB, 98%, C 31 H 48 O 2 S 2 ), low viscosity sodium alginate (99%) and cholic acid (CA, 98%, C 24 H 40 O 5 ) were purchased from Sigma Chemical Co, USA. Calcium chloride dihydrate (CaCl 2 .2H 2 O, 98%) was obtained from Scharlab S.L, Australia. All solvents and reagents were supplied by Merck (Australia), and were of HPLC grade and used without further manipulation. Dulbecco's Modified Eagle's medium (DMEM) was obtained from Sigma Chemical Co, USA. Mouse ultrasensitive insulin ELISA was purchased from Mercodia (Uppsala, Sweden).
Stock suspensions of PB (10 mg/ml) and CA (1 mg/ml) were prepared by adding the powder to 10% Ultra watersoluble gel. The CaCl 2 stock solution (2%) was prepared by adding CaCl 2 powder to HPLC water. All preparations were mixed thoroughly at room temperature for 6 h, stored in the refrigerator, and used within 48 h of preparation.

Mooranian A., Negrulj R., Arfuso F, & Al-Salami H. (2016). Multicompartmental, multilayered probucol microcapsules for diabetes mellitus: Formulation characterization and effects on production of insulin and inflammation in a pancreatic β-cell line. Artificial cells, nanomedicine, and biotechnology, 44(7).

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