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Protein a alexa fluor 488

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Protein A Alexa Fluor 488 is a fluorescently labeled protein A used for detection and quantification of immunoglobulins. It binds to the Fc region of immunoglobulins, enabling visualization and analysis of antibody-containing samples.

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Lab products found in correlation

Kir2dl3 fc chimera, by R&D Systems (2 mentions) Kir2dl2 fc, by R&D Systems (2 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Fluorescent mounting medium, by Leica (1 mentions) Tirf microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Cryoembedding medium, by Thermo Fisher Scientific (1 mentions) Axioskop2 mot plus microscope, by Zeiss (1 mentions) Alexa 594 donkey anti rat, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Alexa Fluor 488 protein A conjugate Alexa Fluor 488 Alexa 488 Alexa Fluors

4 protocols using protein a alexa fluor 488

1

Antigen Expression and Tumor Targeting

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Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of F9, C51 and WEHI-164 stained with F8-F8-IL15, F8-F8-SD-IL15, KSF-KSF-IL15 and KSF-KSF-SD-IL15 (final concentration 0.01mg/mL) and detected with Protein A-AlexaFluor488 (Invitrogen P11047). A rat anti-mouse CD31 (BD 550274) was used in order to detect blood vessels and detected with donkey anti-rat ALEXA 594 (Invitrogen A21209). Slides were mounted with fluorescent mounting medium containing DAPI (Dako Omnins GM304) and analyzed with Axioskop2 mot plus microscope (Zeiss). For ex vivo immunofluorescence analysis, 129/SvEv mice bearing F9 teratocarcinomas were injected intravenously with 100 μg of F8-F8-IL15, F8-F8-SD-IL15, KSF-KSF-IL15 and KSF-KSF-SD-IL15. After 24 hours mice were sacrificed, tumors and organs were excised and embedded in cryo-embedding medium (Thermo Scientific). Cryostat section of 8 μm were stained with Protein A-AlexaFluor488 (Invitrogen P11047) and donkey anti-rat ALEXA 594 in order to detect the blood vessels stained with rat anti-mouse CD31 (BD 550274). Slides were mounted with fluorescent mounting medium containing DAPI (Dako Omnins GM304) and analyzed with Axioskop2 mot plus microscope (Zeiss).
Corbellari R., Stringhini M., Mock J., Ongaro T., Villa A., Neri D, & De Luca R. (2021). A novel antibody-interleukin-15 fusion protein selectively localizes to tumors, synergizes with TNF-based immunocytokine and inhibits metastasis. Molecular cancer therapeutics, 20(5), 859-871.
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2

Fluorescent Immunodetection of EDA

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EDA expression was assessed on ice-cold acetone fixed 8-μm cryostat sections of F9 teratocarcinoma stained with F8(scDb)-mIL21 (final concentration 0.05 mg/mL) and detected with protein A-AlexaFluor488 (Invitrogen P11047). A rat anti-mouse CD31 (BD 550274) was used to detect blood vessels and revealed with donkey anti-rat ALEXA 594 (Invitrogen A21209). Omission of F8(scDb)-mIL21 was used as negative control. Cell nuclei were stained with DAPI (Invitrogen D1306). Slides were mounted with Dako fluorescent mounting medium and analysed with a Leica TIRF microscope.
Di Nitto C., Neri D., Weiss T., Weller M, & De Luca R. (2022). Design and Characterization of Novel Antibody-Cytokine Fusion Proteins Based on Interleukin-21. Antibodies, 11(1), 19.
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3

KIR-Fc Chimera Binding Assay

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KIR2D2L2‐IgG and KIR2DL3‐IgG fusion constructs (KIR2DL2‐Fc & KIR2DL3‐Fc Chimera; R&D Systems) were conjugated with protein A Alexa Fluor 488 (Invitrogen) at a molar ratio of 6:1. A total of 1 × 106 721.174 cells were incubated with 10 μM peptide as described above and then stained with KIR‐Fc. After fixation in 4% w/v paraformaldehyde cells were analyzed by flow cytometry.
Cassidy S., Mukherjee S., Myint T.M., Mbiribindi B., North H., Traherne J., Mulder A., Claas F.H., Purbhoo M.A., Das J, & Khakoo S.I. (2014). Peptide selectivity discriminates NK cells from KIR2DL2‐ and KIR2DL3‐positive individuals. European Journal of Immunology, 45(2), 492-500.
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4

KIR2DL2-Fc and KIR2DL3-Fc Binding Assay

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KIR2D2L2-IgG and KIR2DL3-IgG fusion constructs (KIR2DL2-Fc & KIR2DL3-Fc Chimera; R&D Systems) were conjugated with protein A Alexa Fluor 488 (Invitrogen) at a molar ratio of 6:1. 1 × 106 721.174 cells were incubated with 10 μM peptide as described above and then stained with KIR-Fc. After fixation in 4% (w/v) paraformaldehyde cells were analyzed by flow cytometry.
Cassidy S., Mukherjee S., Myint T.M., Mbiribindi B., North H., Traherne J., Mulder A., Claas F.H., Purbhoo M.A., Das J, & Khakoo S.I. (2014). Peptide selectivity discriminates NK cells from KIR2DL2‐ and KIR2DL3‐positive individuals. European Journal of Immunology, 45(2), 492-500.
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