Logarithmically growing cells of interest were collected and seeded into 6-well plates (500 cells per well) and cultured in an incubator for 14 days. After the incubator culture was completed, the original medium was discarded. The 4% paraformaldehyde (Meilunbio, China), 0.1% crystal violet staining solution (Meilunbio, China) and PBS were used to fix, stain, and wash. Subsequently, the 6-well plate was placed in a fume hood to dry. Colonies larger than 50 cells were counted. Three independent experiments were performed in each experimental group.
Crystal violet staining solution
Manufactured by Meilun
Sourced in China
Crystal violet staining solution is a laboratory reagent used for staining biological samples. It contains a dye called crystal violet, which binds to cell walls and nucleic acids, enabling visualization of cellular structures under a microscope.
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Lab products found in correlation
2 protocols using crystal violet staining solution
1
Clonogenic Assay for Cell Proliferation
2
Cell Migration Assay Protocol
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Cells (2 × 105) were suspended in serum-free medium and seeded into the inserts of a 24-well Transwell chamber (Corning, NY, USA) in triplicate. The inserts were co-cultured on a 24-well plate containing 10% FBS as an attractant. After incubation at 37 °C in a 5% CO2 atmosphere for 24 h, the inserts were removed, and the upper surface of the membrane was scrubbed to remove the non-migrating cells. The inserts were then fixed with 4% paraformaldehyde and stained with crystal violet staining solution (meilunbio, Dalian, China). Images of migrated cells were acquired, and the average cell counts were calculated.
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