Human fibroblasts (WS-1) cell line and breast cancer (MCF-7) cell lines were purchased from National Centre for Cell Sciences (NCCS) (Pune, India). The cells were preserved in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum and penicillin/streptomycin under 5% CO2 atmosphere at 37 °C.
Mcf 7 cell line
Manufactured by National Centre for Cell Science
Sourced in India
MCF-7 is a human breast adenocarcinoma cell line. It is a commonly used model for studying breast cancer biology and evaluating the efficacy of potential cancer therapies.
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6 protocols using mcf 7 cell line
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Cell Culture of Human Fibroblast and Breast Cancer
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Culturing Human Breast Cancer Cells
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Human breast cancer (MCF-7) cell lines were procured from National Centre for Cell Sciences (NCCS), Pune, India. The cell line was maintained in complete Dulbecco’s Modified Eagle Medium (DMEM) in the humidified incubator with 5% CO2 in 37 °C in T25 flasks. Complete medium means DMEM supplemented with 10% (v/v) FBS, 100 IU/mL penicillin, 100 µg/mL streptomycin and 2.5 µg/mL amphotericin.
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Cultivation of Breast Cancer MCF-7 Cells
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Breast cancer (MCF-7) cell lines were obtained from the National Centre for Cell Science (NCCS), Pune. The cells were grown in T25 culture flasks containing dulbecco's modified eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and amphotericin B 3 m/L, streptomycin 75 mg/L, gentamycin 180 mg/L, penicillin 120 mg/L, cells were maintained at 37ºC in a humidified atmosphere containing 5% CO .
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Culturing Human Breast Cancer Cells
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Human breast adenocarcinoma (MCF-7) cell line was purchased from the National Centre for Cell Science (NCCS), India and maintained in sterilized condition. The cells were grown in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin G, 50 μg/ml gentamycin sulphate, 100 μg/ml streptomycin and 2.5 μg/ml amphotericin-B. The cell line was maintained at 37°C in a humidified atmosphere containing 5% CO2.
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MCF-7 Cell Line Maintenance and Cytotoxicity
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The MCF-7 cell line (human breast cancer epithelial cells) was obtained from the National Centre for Cell Science, Pune, India. For the culture maintenance and in vitro cytotoxicity experiment, MCF-7 cells were cultured in a culture flask or on plates (Falcon, Sigma-Aldrich, Bengaluru, India) in DMEM, supplemented with 20% heat inactivated FBS, 1% penicillin, streptomycin, and amphotericin B in stable conditions of 37 °C and 5% CO2 in a humidified atmosphere. A passage no higher than 20 was used (P18 in most of the experiments). The cells were frequently tested against mycoplasma according to the laboratory protocol [27 (link)].
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Antimicrobial and Antioxidant Activity of A. concinna and C. limon
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A. concinna and C. limon used in the present investigation were collected from the University of Agriculture Science (Dharwad, India), and brought to the biochemistry laboratory. The bacterial strains used were B. cereus, E. coli, Z. mobilis, B. subtilis, M. luteus, P. aeruginosa, and S. aureus. They were obtained from the Microbial Type Culture Collection (Chandigarh, India). The MCF-7 cell line was bought from the National Centre for Cell Sciences (Pune, India). The chemicals such as DMSO, DPPH, MTT, BSA, p-nitrophenyl palmitate, etc., were bought from Himedia (New Delhi, India) and Sigma-Aldrich Pvt Ltd. (St. Louis, MO, USA), Bangalore, Karnataka. All the reagents utilized in the present study were of analytical grade.
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