Phenogel column

Manufactured by Waters Corporation

Sourced in United States

Phenogel™ column is a size exclusion chromatography (SEC) column designed for the analysis of polymers, oligomers, and macromolecules. It provides efficient separation and high-resolution performance for a wide range of molecular weights.

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Lab products found in correlation

Ultraflextreme mass spectrometer, by Bruker (1 mentions) 0.45 μm filter, by Cytiva (1 mentions) Chloroform, by Merck Group (1 mentions) Model 410, by Waters Corporation (1 mentions) Gpc system, by Waters Corporation (1 mentions) Whatman, by Cytiva (1 mentions)

2 protocols using phenogel column

Characterization of mPEG-Polymer Samples

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Proton nuclear magnetic resonance spectra (¹H-NMR) were obtained using a Bruker spectrometer at 400 MHz. The samples were dissolved in CDCl₃. Chemical shifts (δ) were expressed in parts per million (ppm) with respect to a tetramethylsilane reference. The molecular weight of the mPEG initiator was evaluated through MALDI-TOF mass spectrometry by using an ultrafleXtreme mass spectrometer (Bruker), and a weight of 5.31 kDa was obtained.
The molecular weight distribution of the polymers was determined through gel permeation chromatography (GPC) by using a 5 µm 1 × 10³ Å Phenogel™ column in a chromatographer (Waters, Pittsburgh, PA, USA). THF was used as the mobile phase (flow rate 0.7 mL/min at 35 °C). Column calibration was performed with polystyrene (PS) standards between 1.5 and 50 kDa.

Arias E.R., Angarita-Villamizar V., Baena Y., Parra-Giraldo C, & Perez L.D. (2021). Phospholipid-Conjugated PEG-b-PCL Copolymers as Precursors of Micellar Vehicles for Amphotericin B. Polymers, 13(11), 1747.

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Molecular Weight Characterization of Polymer Samples

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Specimens were dissolved in chloroform (Sigma-Aldrich, St. Louis, MO) at a concentration of 20 mg/mL under mild agitation at room temperature and filtered by injection through a 0.45 μm filter (Whatman, Kent, UK). The resulting solution was assayed for molecular weight by gel permeation chromatography (GPC) based on previously reported methods [17 (link), 24 ]. The GPC system (Waters, Milford, MA) consisted of a pump (Waters Model # 155), injection module (Waters # 717), and refractive index detector (Waters Model # 410). GPC was performed (n = 3) at a flow rate of 1 mL/min and temperature of 30 °C using a Phenogel column (300 × 7.80 mm, 5 μm particle size, Waters) and calibrated using linear PMMA standards with molecular weights ranging from 2.58 to 981 kDA (Waters, Milford, MA). Number average molecular weight (M_n), weight average molecular weight (M_w), and polydispersity index (PI) were measured and reported.

Tatara A.M., Rozich A.J., Kontoyiannis P.D., Watson E., Albert N.D., Bennett G.N, & Mikos A.G. (2018). Econazole-releasing porous space maintainers for fungal periprosthetic joint infection. Journal of materials science. Materials in medicine, 29(5), 70.

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