Log In Sign up
The largest database of trusted experimental protocols

Taqman fast reagents

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Taqman Fast Reagents are a set of reagents designed for use in real-time PCR (polymerase chain reaction) assays. These reagents are optimized for fast thermal cycling protocols, enabling rapid and efficient amplification of target DNA sequences.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (4 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Cfx96 real time pcr system, by Bio-Rad (2 mentions) Qscript xlt cdna supermix, by Quantabio (2 mentions) Ezna total rna kit extraction kit, by Omega Bio-Tek (2 mentions) Taqman probe, by Thermo Fisher Scientific (2 mentions) Amv reverse transcription system, by Promega (1 mentions) Qscript cdna supermix, by Quantabio (1 mentions) Gapdh mm99999915 g1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

TaqMan™ Fast Advanced reagents TaqMan Universal Fast PCR master mix reagents TaqMan reagents

5 protocols using taqman fast reagents

1

Quantifying HCV Viral Load by RT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
rt‐PCR was performed with the TaqMan fast 7500 system using TaqMan fast reagents (Thermo Fisher Scientific). An rt‐PCR targeting the 5′ UTR was used to quantify the HCV viral load21 using a dilution series prepared from known concentrations of JFH‐1 replicon transcripts. Genotype‐specific rt‐PCR was performed using newly designed primers and probes targeting the NS5B region (Table 1).
McNaughton A.L., Sreenu V.B., Wilkie G., Gunson R., Templeton K, & Leitch E.C. (2018). Prevalence of mixed genotype hepatitis C virus infections in the UK as determined by genotype‐specific PCR and deep sequencing. Journal of Viral Hepatitis, 25(5), 524-534.
+ Open protocol
+ Expand
2

Intestinal mRNA Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For mRNA extraction, intestinal samples from jejunum and colon were homogenized in TRIzol (Invitrogen, Carlsbad, CA) and isolated according to manufacturer’s protocols. mRNA quality and concentrations were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA). Complementary DNA was generated using the AMV reverse transcription system (Promega, Madison, MI) following manufacturer’s protocols. Expression of inflammatory, metabolic, and gut health markers were determined via quantitative qPCR utilizing Taqman fast reagents (Thermo Scientific, Waltham, MA) in an CFX96 Real-Time PCR system (Bio-Rad, Hercules, CA). β-actin was used as the housekeeping gene for jejunum and 18S was used as the housekeeping gene for colon. Differences in gene expression were calculated using ΔΔCt relative quantification compared to control animals.
Petriello M.C., Hoffman J., Vsevolozhskaya O., Morris A.J, & Hennig B. (2018). Dioxin-like PCB 126 increases intestinal inflammation and disrupts gut microbiota and metabolic homeostasis. Environmental pollution (Barking, Essex : 1987), 242(Pt A), 1022-1032.
+ Open protocol
+ Expand
3

Mouse Tissue RNA Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated from various mouse tissues (30 mg), homogenized, and then extracted using EZNA Total RNA Kit extraction kit (Omega Bio‐tek, Norcross, GA). The complementary DNA was generated from 2 μg of RNA using qScript XLT cDNA Supermix (Quantabio, Beverly, MA). Real‐time reverse transcriptase polymerase chain reaction was performed with TaqMan probes (Thermo Fisher Scientific, Waltham, MA) and TaqMan Fast reagents. Reactions were carried out in triplicate and normalized to glyceraldehyde 3‐phosphate dehydrogenase.
Fu Q., Chen M., Anderson J.T., Sun X., Hu S., Sparreboom A, & Baker S.D. (2019). Interaction Between Sex and Organic Anion‐Transporting Polypeptide 1b2 on the Pharmacokinetics of Regorafenib and Its Metabolites Regorafenib‐N‐Oxide and Regorafenib‐Glucuronide in Mice. Clinical and Translational Science, 12(4), 400-407.
+ Open protocol
+ Expand
4

Hepatic mRNA Expression Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver samples were homogenized in TRIzol (Invitrogen, Carlsbad, CA) and mRNA was extracted according to manufacturer’s instructions. Quality and concentrations were quantitated using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA) as conducted in previous studies (Petriello, 2018 (link); Petriello et al., 2018 (link); Wahlang et al., 2017a (link)). Complimentary DNA was generated utilizing qScript cDNA SuperMix with genomic DNA wipeout (Quantabio, Beverly, MA). Gene expression was determined via qPCR utilizing Taqman fast reagents (Thermo Scientific, Waltham, MA) in a CFX96 Real-Time PCR system (Bio-Rad, Hercules, CA). The Taqman primers used were Tnfa (Mm00443258_m1), Lbp (Mm00493139_m1), Tlr4 (Mm00445273_m1), Mrp2 (Mm00445273_m1), Pklr (Mm00443090_m1), Gck (Mm00439129_m1), Pck1 (Mm00439129_m1), G6pc (Mm00839363_m1) 18S was used as the housekeeping gene after determination that expression values varied fairly tightly in line with Actb measures and based on previous validated use in a similar animal model (Petriello et al., 2016 (link)). The ΔΔCt relative quantification method was used to calculate fold differences in gene expression.
Hoffman J.B., Petriello M.C., Morris A.J., Mottaleb M.A., Sui Y., Zhou C., Deng P., Wang C, & Hennig B. (2020). Prebiotic inulin consumption reduces dioxin-like PCB 126-mediated hepatotoxicity and gut dysbiosis in hyperlipidemic Ldlr deficient mice. Environmental pollution (Barking, Essex : 1987), 261, 114183.
+ Open protocol
+ Expand
5

Isolation and Quantification of Cardiac and Renal RNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated from adult or neonatal wild-type hearts and kidneys (30 mg) as well as from cardiomyocyte cells isolated from wild-type and MATE1-deficient mice. Tissues and cardiomyocytes were homogenized and then RNA was extracted using an EZNA Total RNA Kit extraction kit (Cat# R6834-02, Omega Bio-tek, Norcross, GA, USA). cDNA was generated from 2 μg of RNA using qScript XLT cDNA Supermix (Cat# 95161-100, Quantabio, Beverly, MA, USA). Real-time reverse transcriptase PCR (RT-PCR) was performed with TaqMan primer (Mm00840361_m1, Thermo Fisher Scientific, Waltham, MA, USA) and TaqMan Fast reagents. Reactions were carried out in triplicate, and normalized to Gapdh (Mm99999915_g1, Thermo Fisher Scientific, Waltham, MA, USA).
Uddin M.E., Eisenmann E.D., Li Y., Huang K.M., Garrison D.A., Talebi Z., Gibson A.A., Jin Y., Nepal M., Bonilla I.M., Fu Q., Sun X., Millar A., Tarasov M., Jay C.E., Cui X., Einolf H.J., Pelis R.M., Smith S.A., Radwański P.B., Sweet D.H., König J., Fromm M.F., Carnes C.A., Hu S, & Sparreboom A. (2022). MATE1 Deficiency Exacerbates Dofetilide-Induced Proarrhythmia. International Journal of Molecular Sciences, 23(15), 8607.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!