Fluconazole

To ablate the mycobiome in mice, animals were administered Amphotericin B (1mg/ml; MP Biomedicals, Santa Ana, CA) by oral gavage daily for five consecutive days in addition to adding Amphotericin B (0.5μg/ml) to mouse drinking water for the duration of the experiment^{10 (link)}. Controls were gavaged with PBS. Orthotopic PDA tumor cells were administered or pancreatitis was initiated 3 weeks after commencing treatment with Amphotericin B. Alternatively, mice were treated with Fluconazole (0.5mg/ml; MP Biomedicals) for 3 weeks prior to tumor implantation using the same regimen^{11 (link)}. For species-specific repopulation experiments, M. globosa (MYA-4612, 1×10⁸ CFU/ml), S. cerevisiae (7752, 1×10⁸ CFU/ml), C. tropicalis (MYA-3404, 1×10⁸ CFU/ml; all ATCC Manassas, VA), Candida sp. (clinical isolate; 1×10⁸ CFU/ml) or Aspergillus sp. (clinical isolate; 1×10⁶ CFU/ml) were used to orally gavage mice following fungal ablation with Amphotericin B. Recipient mice were administered orthotopic PDA cells 7 days after repopulation. To assess fungal translocation to the pancreas, 10⁸ CFU of GFP-labeled Saccharomyces cerevisiae (ATCC MYA-2011) were introduced via oral gavage, and pancreatic samples were examined at 30 minutes by flow cytometry. All experiments were approved and in compliance with the New York University School of Medicine Institutional Animal Care and Use Committee (IACUC).