TRPL was performed in ambient air based on an FLSP920 Fluorescence Spectrometer (Edinburgh Instruments Ltd.) using the TCSPC acquisition technique. A picosecond pulsed laser diode (PicoQuant, 635 nm) externally triggered by a delay generator (repetition rate: 500 kHz) was used as the excitation light. The emission was collected by a photomultiplier tube (Hamamatsu R928P).
Flsp920 fluorescence spectrometer
Manufactured by Edinburgh Instruments
Sourced in United Kingdom
The FLSP920 is a fluorescence spectrometer designed for accurate and reliable measurement of fluorescence spectra. It features a high-performance monochromator, sensitive detectors, and advanced control software for precise data acquisition and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Avance drx 500, by Bruker (1 mentions)
Drx 300, by Bruker (1 mentions)
Q tof premier mass spectrometer, by Waters Corporation (1 mentions)
Evolution 60, by Thermo Fisher Scientific (1 mentions)
Digital micrograph software, by Ametek (1 mentions)
S 4700, by Hitachi (1 mentions)
Tecnai g2 f20, by Thermo Fisher Scientific (1 mentions)
Uv 2600 spectrophotometer, by Shimadzu (1 mentions)
S 4800, by Hitachi (1 mentions)
Escalab 250 spectrometer, by Thermo Fisher Scientific (1 mentions)
D8 advance x, by Bruker (1 mentions)
3flex apparatus, by Micromeritics (1 mentions)
Phi 5000c esca system, by PerkinElmer (1 mentions)
Fv1000 confocal laser microscope, by Olympus (1 mentions)
6 protocols using flsp920 fluorescence spectrometer
1
Time-Resolved Photoluminescence Characterization
2
Synthesis and Characterization of 5-Chloromethyluracil
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents, reagents and starting materials were obtained from commercial suppliers and used without further purification. 5-Chloromethyluracil [63 (link)] was synthesized as described previously. The fluorescence spectra were measured on an Edinburgh Instruments FLSP 920 fluorescence spectrometer. The 1H NMR spectra were taken on a Bruker Avance DRX-500 or DRX-300 spectrometer with chemical shifts reported in ppm (TMS in in the case of CDCl3 and the residual DMSO in the case of DMSO-d6 was used as internal standard). The exact mass measurements were performed using a Q-TOF Premier mass spectrometer (Waters Corporation, 34 Maple St, Milford, MA, USA) using electrospray ionization in positive mode.
3
Curcumin Liposomes: Synthesis and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The synthesis and characterization of curcumin liposomes involved several steps and equipment. A Stuart rotary evaporator (RE300) was used to initiate the synthesis process. To obtain unilamellar vesicles, the multilamellar liposomes were subjected to sonication using an Elma D-78224 sonication bath (Melrose Park, IL, USA). Afterward, extrusion with an Avanti Polar Lipids extruder (Alabaster, USA), resulted in uniform-sized curcumin liposomes (CL). The curcumin liposomes' size and other structural properties (CL) were analyzed using the dynamic light scattering method with a Dyanopro Nanostar instrument (Wyatt Technology Corp., Santa Barbara, CA, USA). Free curcumin and CL's fluorescence emission and excitation behavior were quantified using an FLSP920 fluorescence spectrometer (Edinburgh Instruments Ltd., Livingston, UK).
Absorbance measurements were made using a UV–visible spectrophotometer (EVOLUTION 60 S, Thermo Scientific, Waltham, Massachusetts, USA). In vitro studies involving 1 MHz ultrasound delivery employed a Precision Acoustics Ultrasonic transducer, continuous mode (Transducer Diameter 2.54 cm, surface area 1.5 cm2) (Dorchester, UK). The Accu Reader plate reader was employed to read 96-well microtiter plates (Accu Reader Metertech, Taiwan). Flow cytometry analysis was carried out using a Beckman Coulter FC500 flow cytometer (Indianapolis, United States).
Absorbance measurements were made using a UV–visible spectrophotometer (EVOLUTION 60 S, Thermo Scientific, Waltham, Massachusetts, USA). In vitro studies involving 1 MHz ultrasound delivery employed a Precision Acoustics Ultrasonic transducer, continuous mode (Transducer Diameter 2.54 cm, surface area 1.5 cm2) (Dorchester, UK). The Accu Reader plate reader was employed to read 96-well microtiter plates (Accu Reader Metertech, Taiwan). Flow cytometry analysis was carried out using a Beckman Coulter FC500 flow cytometer (Indianapolis, United States).
4
Comprehensive Characterization of Novel Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
X-ray diffraction (XRD) patterns were recorded on a Bruker D8 Advance X-ray diffractometer using a Cu Ka radiation source (k = 1.5406 Å). The general morphology of prepared samples was observed by means of scanning electron microscopy (SEM, Hitachi S-4800 and Hitachi S-4700). TEM, HRTEM patterns, and SAED images were recorded on a FEI Tecnai G 2 F20 transmission electron microscope operated at 200 kV. HRTEM was analyzed using the Digital Micrograph software (Gatan Inc.). X-ray photoelectron spectroscopy (XPS) measurements were performed by using a RBD upgraded PHI 5000C ESCA System (Perkin Elmer) with Mg Ka (1253.6 eV) radiation. Binding energies were calibrated by using the containment carbon (C1s = 284.6 V). The valence-band X-ray photoelectron spectroscopy (VB XPS) spectrum was obtained on a Thermo ESCALAB 250 spectrometer using a monochromated Al-Ka source (1486.6 eV). The specific surface areas were measured using the BET method by Nitrogen adsorption-desorption isotherms at 77 K using a Micromeritics 3Flex apparatus. Before measurement, the samples were degassed at 383 K under vacuum for more than 12 h. UV-vis diffuse reflectance spectra (UV-Vis DRS) were obtained on a Shimadzu UV-2600 spectrophotometer using BaSO 4 as a reference. Photoluminescence (PL) spectra were measured with Edinburgh FLSP920 fluorescence spectrometer with an excitation wavelength of 324 nm.
5
Characterization of Novel Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1H-NMR and 13C-NMR spectra were recorded on a JEOL-ECS-400MHz (JEOL Ltd., Tokyo, Japan) using tetramethylsilane (TMS) as an internal standard. Mass spectra were obtained by a Thermo LTQ Orbitrap XL Mass spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) and a LQC system (Finngan MAT, San Jose, CA, USA). UV-Vis absorption spectra were recorded on a TU-1810 spectrophotometer (PEXI, Beijing, China). The OP excited fluorescence spectra were measured out on a RF-5310PC spectrofluorophotometer (Shimadzu, Tokyo, Japan) and TP excited fluorescence spectra and the fluorescent quantum yield were determined on a FLSP920 fluorescence spectrometer (Edinburgh Instruments Ltd., Livingston, UK). TP fluorescence images were recorded by an Olympus FV1000 laser confocal microscope. The pH values were calibrated with a model 868 pH meter (Leici, Shanghai, China). Unless otherwise noted, materials from commercial suppliers were used without further purification.
6
Fluorescence Titration of TAR RNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All measurements were carried in a Edinburgh Instruments FLSP920 fluorescence spectrometer (λex 545 nm, λem 600 nm, dwell time 0.5 s) using a 3 mL quartz cuvette. The emission of TAR RNA (0.3 μM in Phosphate Buffer, pH 7.0, previously annealed as above) in the presence of Ethidium Bromide (EB, 1.2 μM) was measured and normalized to 0% fluorescence. Increasing concentrations of cylinders were added (from 0.1 to 2 equivalents in respect to TAR RNA) allowing 10 min stabilization before measuring emission. Each titration was repeated 3 times, reported as normalized variation of emission at 600 nm (ΔF) vs Cylinder/TAR ratio (Figure S2 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!