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3 protocols using calcium chloride

1

Breadfruit-based Bioactive Formulation

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Breadfruit was purchased from a local market in Owerri, Imo State, Nigeria. Theophylline was obtained from Wuhan Hezhong Bio-Chemical Manufacture Co., Ltd., Wuhan city, China. Sodium alginate was obtained from S.D. Fine Chem (Mumbia, India). Chitosan was obtained from Sigma-Aldrich, St Louis, MO, USA. Calcium chloride was obtained from Loba Chemie Pvt. Ltd., Mumbai, India.
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2

Cellulose Extraction from Water Hyacinth

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Cellulose was extracted from water hyacinth; acrylic acid and N,N-methylene–bis-acrylamide were obtained from ACROS Organics, Germany. Ammonium persulphate, acetone, toluene, ammonium hydroxide, hydrochloric acid, sulphuric acid, sodium hypochlorite, sodium chloride, calcium chloride and aluminium chloride were obtained from Loba Chemie, Mumbai, India.
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3

Breadfruit-Based Theophylline Alginate Beads

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Breadfruit was bought from a local market in Owerri, Imo State (latitude of 5.476310, and longitude of 7.025853). The materials used in this study include: theophylline (Wuhan Hezhong Bio-Chemical Manufacture Co., Ltd. China), sodium alginate (S.D. Fine Chem Mumbai, India), calcium chloride (Loba Chemie Pvt. Ltd., Mumbai, India) All other chemicals used were of analytical grade.
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