Pf 3758309

Manufactured by Chemietek

The PF-3758309 is a laboratory equipment designed for precise liquid handling. It features an adjustable volume range and digital display for accurate measurements. The device is built with high-quality materials to ensure durability and reliable performance.

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Lab products found in correlation

C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Colo205, by American Type Culture Collection (1 mentions) Azd6244, by Selleck Chemicals (1 mentions) Ipa 3, by Selleck Chemicals (1 mentions) Sk mel 28, by American Type Culture Collection (1 mentions) Ht 29, by American Type Culture Collection (1 mentions) Vemurafenib, by LC Laboratories (1 mentions) Mycoalert kit, by Lonza (1 mentions)

2 protocols using pf 3758309

Characterization of Pmp22+/- Mice and PAK1 Inhibitor Study

Cited 2 times

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Pmp22+/- mice were backcrossed with C57Bl6J mice (Jackson Lab) for more than 10 generations to reach congenic. Mice were genotyped as described [9 (link)]. The primers for genotyping are listed in S3 Table. The congenic Pmp22+/- mice have been extensively characterized [5 (link)]. They showed pathology and other features similar to those in Pmp22+/- mice with mixed background (C57Bl6J/129) [10 (link)].
Pak1-/- mice were from Dr. Jonathan Chernoff’s lab, Fox Chase Cancer Center, USA. The Pak1-/- mice were produced in C57Bl6J background and have been described with negligible phenotype [19 (link)].
For PAK1 inhibitor (PF3758309) injections, Pmp22+/- mice were randomized into vehicle and treated groups. Based on our power calculation of variations derived from mouse compound nerve action potentials, 7 mice for each group would have a 92% chance to detect a significant difference. PF-3758309 (Cat# CT-PF0375, ChemieTek) was dissolved in normal saline for intraperitoneal injection (i.p.) daily.

Hu B., Arpag S., Zhang X., Möbius W., Werner H., Sosinsky G., Ellisman M., Zhang Y., Hamilton A., Chernoff J, & Li J. (2016). Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP. PLoS Genetics, 12(9), e1006290.

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Cell Line Authentication and Validation

Cited 1 time

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All cell lines were cultured in humidified incubators at 37°C in the presence of 5% CO₂. A375 (obtained from ATCC), HT29 (a gift from Dr. Yuri Ionov) and Colo205 (obtained from ATCC) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM). SK-MEL-28 (obtained from ATCC) were maintained in Minimum Essential Media (MEM). B-CPAP (a gift from Dr. Katerina Gurova) were maintained in RPMI1640. All culture media were supplemented with penicillin (100 U/ml), streptomycin (100 µg/ml) and fetal bovine serum (10%).
The cells were authenticated by the Roswell Park Cancer Research Institute’s Genomics Shared Resource using Amp FLSTR Identifier Plus PCR Amplification Kit (A26182; ThermoFisher Scientific).
The cells were free of mycoplasma (as assessed by MycoAlert kit from Lonza, Inc.) and contaminating gamma-retroviruses [41 (link)].
PF3758309 was purchased from ChemieTek. Vemurafenib was obtained from LC Laboratories. IPA3 and AZD6244 were obtained from Selleck Chemicals.

Babagana M., Johnson S., Slabodkin H., Bshara W., Morrison C, & Kandel E.S. (2017). P21-Activated Kinase 1 Regulates Resistance to BRAF Inhibition in Human Cancer Cells. Molecular carcinogenesis, 56(5), 1515-1525.

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