Identical levels of proteins were separated by SDS-PAGE and analyzed by western-blot with the indicated antibodies as described previously [20 (link), 21 (link)]. Antibodies used for western blot: cleaved-PARP, cleaved-caspase 7, cleaved-caspase 8, cleaved-caspase 9, BIM, phospho-JNK, phospho-AKT, phospho-BAD-ser136, phospho-Foxo-3a-ser253, phospho-ERK, phospho-BAD-ser112, (Cell Signaling), tubulin(SIGMA), and Actin (Millipore). Quantifications were performed with ImageJ software.
Phospho bad ser136
Manufactured by Cell Signaling Technology
Phospho-BAD(Ser136) is an antibody that detects the phosphorylation of the BAD protein at serine 136. BAD is a pro-apoptotic member of the Bcl-2 family of proteins that plays a role in regulating cell survival and apoptosis. Phosphorylation of BAD at serine 136 inactivates its pro-apoptotic function.
Automatically generated - may contain errors
Lab products found in correlation
Phospho akt, by Cell Signaling Technology (1 mentions)
Phospho erk, by Cell Signaling Technology (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Phospho bad ser112, by Cell Signaling Technology (1 mentions)
Phospho foxo 3a ser253, by Cell Signaling Technology (1 mentions)
β actin a5441, by Merck Group (1 mentions)
Nox4 nb110 58849, by Novus Biologicals (1 mentions)
Phospho akt1, by Cell Signaling Technology (1 mentions)
Vdac 4866, by Cell Signaling Technology (1 mentions)
2 protocols using phospho bad ser136
1
Protein Expression Analysis by Western Blot
2
Apoptosis Signaling Pathway Profiling
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Primary antibodies used: Akt1 (2938), Bad (9239), Lamin A/C (2032), Bax (2772), phospho-Akt1 (9018), phospho-Bad (Ser136) (4366), VDAC (4866) (Cell Signaling); β-actin (A5441) (Sigma); p38 (sc-7972) (Santa Cruz); NOX4 (NB110-58849) (Novus), NOX4 (109225) (Abcam); Bak (A0404) (Abclonal).
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