Total RNA was isolated from total testis using Ribozol, and RNA was treated with DNase. For library cloning, 1 μg DNase-treated RNA was poly(A)+ selected and RNA-sequencing (RNA-seq) libraries were cloned using either Illumina TruSeq RNA sample prep kit (version 2, 48 rxns, RS-122-2001) or NEB Next Ultra RNA library prep kit for Illumina (New England Biolabs, E7530). Small RNAs (19–30 nt) were extracted from total RNA by separation on 12% polyacrylamide gel and cloned as described elsewhere (Pastor et al., 2014 (link)); for a detailed protocol, see Supplemental Experimental Procedures.
Truseq rna sample prep kit
Manufactured by New England Biolabs
The TruSeq RNA sample prep kit is a laboratory equipment product designed for the preparation of RNA samples for sequencing. It provides a standardized workflow for RNA extraction, purification, and conversion to cDNA, preparing the samples for downstream sequencing analysis.
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2 protocols using truseq rna sample prep kit
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Testis Total RNA Extraction and Sequencing
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Testis Total RNA Extraction and Sequencing
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Total RNA was isolated from total testis using Ribozol, and RNA was treated with DNase. For library cloning, 1 μg DNase-treated RNA was poly(A)+ selected and RNA-sequencing (RNA-seq) libraries were cloned using either Illumina TruSeq RNA sample prep kit (version 2, 48 rxns, RS-122-2001) or NEB Next Ultra RNA library prep kit for Illumina (New England Biolabs, E7530). Small RNAs (19–30 nt) were extracted from total RNA by separation on 12% polyacrylamide gel and cloned as described elsewhere (Pastor et al., 2014 (link)); for a detailed protocol, see Supplemental Experimental Procedures.
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