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Wm 115

Manufactured by Thermo Fisher Scientific
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The WM-115 is a water bath designed for laboratory use. It maintains a stable temperature within a specified range to support various experimental and testing procedures. The unit provides consistent temperature control through its integrated heating and circulation system.

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Lab products found in correlation

Wm115, by American Type Culture Collection (4 mentions) Sk mel 28, by Thermo Fisher Scientific (4 mentions) Sk mel 28, by American Type Culture Collection (4 mentions) Sodium pyruvate, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Wm1552c, by American Type Culture Collection (2 mentions) Mz7mel, by RIKEN BioResource Center (2 mentions) Mmacsf, by Thermo Fisher Scientific (2 mentions) Mmacsf, by RIKEN BioResource Center (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Wm3211, by Rockland Immunochemicals (2 mentions) Wm3211, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Lentivirus, by Hanbio Biotechnology (2 mentions) Hek293t, by American Type Culture Collection (1 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Rpmi 1640, by Corning (1 mentions)

8 protocols using wm 115

1

Cell Culture Conditions for Melanoma and HEK293T

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Cell lines used in this study were obtained from the Massachusetts General Hospital Cancer Center and originated from the following primary sources: A375, C32, K2, RVH421, WM115, SKMEL28, and WM1552C (ATCC);MMACSF (RIKEN BioResource Center); and MZ7MEL (Johannes Gutenberg University Mainz). HEK293T was from ATCC. C32, K2,MMACSF, SKMEL28, RVH421, and WM115 cell lines were grown in DMEM/F12 (Invitrogen) supplemented with 5% heat inactivated fetal bovine serum (FBS) (Gibco) and 1% sodium pyruvate (Invitrogen). MZ7MEL and WM1552C were grown in RPMI-1640 (Corning) supplemented with 5% FBS and 1% sodium pyruvate (Invitrogen). A375 cells were grown in Dulbecco’s modified eagle medium with 4.5 g/l D-glucose, 4 mM L-glutamine, and 1mM sodium pyruvate (DMEM) (Corning), supplemented with 5% FBS. HEK293T cells were grown in DMEM supplemented with 10% FBS. Penicillin and streptomycin were added to all growth media at final concentrations of 100 U/mL and 100 μg/mL, respectively (Corning). Cells were tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza).
Gerosa L., Chidley C., Fröhlich F., Sanchez G., Lim S.K., Muhlich J., Chen J.Y., Vallabhaneni S., Baker G.J., Schapiro D., Atanasova M.I., Chylek L.A., Shi T., Yi L., Nicora C.D., Claas A., Ng T.S., Kohler R.H., Lauffenburger D.A., Weissleder R., Miller M.A., Qian W.J., Wiley H.S, & Sorger P.K. (2020). Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells. Cell systems, 11(5), 478-494.e9.
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2

Melanoma Cell Line Cultivation Protocol

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Melanoma cell lines used in this study were obtained from the Massachusetts General Hospital Cancer Center with the following primary sources: COLO858 (ECACC), A375, C32, WM115, SKMEL28, and WM1552C (ATCC), LOXIMV1 (DCTD Tumor Repository, National Cancer Institute), MMACSF (RIKEN BioResource Center), and MZ7MEL (Johannes Gutenberg University Mainz). C32, MMACSF, SKMEL28, and WM115 cell lines were grown in DMEM/F12 (Invitrogen) supplemented with 5% fetal bovine serum (FBS) and 1% sodium pyruvate (Invitrogen). COLO858, LOXIMVI, MZ7MEL, and WM1552C cell lines were grown in RMPI 1640 (Corning cellgro) supplemented with 5% FBS and 1% sodium pyruvate (Invitrogen). A375 cells were grown in DMEM with 4.5 g/l glucose, l‐glutamine, and sodium pyruvate (Corning cellgro), supplemented with 5% FBS. We added penicillin (50 U/ml) and streptomycin (50 μg/ml) to all growth media.
Fallahi‐Sichani M., Becker V., Izar B., Baker G.J., Lin J., Boswell S.A., Shah P., Rotem A., Garraway L.A, & Sorger P.K. (2017). Adaptive resistance of melanoma cells to RAF inhibition via reversible induction of a slowly dividing de‐differentiated state. Molecular Systems Biology, 13(1), 905.
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3

Comprehensive Cell Line Characterization

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Pancreatic cancer cells (MIA PaCa-2, PANC-1, and BxPC-3) and colon cancer cells (HCT116 p53+/+, HT29) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Human Pancreatic Nestin-expressing (HPNE) and UM59 (22 (link)) cells were kindly provided by Dr. Diane Simeone (University of Michigan, Ann Arbor, MI, USA). Other cell lines, HCT116 p53−/− and H1299 were obtained from John Hopkins, A549, MCF-7, OVCAR-8, OVCAR-3, and Skov-3 were obtained from NCI, SHEP-1 and SHSY-5Y were obtained from Erika Newman, U of M, WM115 was obtained from Fallahi-Sichani Lab, U of M. Cell lines were maintained in the appropriate growth media containing 10% heat-inactivated FBS (Gibco) at 37 °C in a humidified atmosphere of 5% CO2. All cell lines used were maintained in culture under 35 (10 for HPNE) passages and tested regularly for mycoplasma contamination using Plasmo Test™ kit (InvivoGen, rep-pt1). Individual cell line authentication was regularly performed twice a year using short tandem repeat (STR) analysis at the institutional core facility.
Samanta S., Yang S., Debnath B., Xue D., Kuang Y., Ramkumar K., Lee A.S., Ljungman M, & Neamati N. (2021). The hydroxyquinoline analog YUM70 inhibits GRP78 to induce ER stress-mediated apoptosis in pancreatic cancer. Cancer research, 81(7), 1883-1895.
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4

Melanoma Cell Lines with Genomic Mutations

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Four human melanoma cell lines carrying distinct genomic mutations (A375: BRAFV600E/PTENWT/CKITWT; Sk-mel-28: BRAFV600E/PTENT167A/CKITWT; wm115: BRAFV600E/PTENWT/CKITWT; and wm3211: BRAFWT/PTENWT/CKITL576P) were used for the study [23 (link),24 ]. A375, wm115, Sk-mel-28, and HEK-293 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA), and wm3211 was obtained from Rockland Immunochemicals (Limerick, PA, USA). Cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, #11995073; Gibco, Waltham, MA, USA) (A375) or Eagle’s Minimum Essential Medium (EMEM) (wm115, Sk-mel-28, HEK-293) with 10% fetal bovine serum (FBS, #26140079; Gibco, Waltham, MA, USA), or Tumor Specialized Media with 2% FBS (wm3211). Cell culture media were supplemented with 10% FBS (Corning, Corning, NY, USA). Cells were cultured in a humidified atmosphere in a 5% CO2 incubator maintained at 37 °C.
Tong S., Darwish S., Ariani H.H., Lozada K.A., Salehi D., Cinelli M.A., Silverman R.B., Kaur K, & Yang S. (2022). A Small Peptide Increases Drug Delivery in Human Melanoma Cells. Pharmaceutics, 14(5), 1036.
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5

Melanoma Tissue Acquisition and Cell Culture

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Fifty-one pairs of malignant melanoma and the tumor adjacent normal tissues were acquired from patients undergoing a surgical procedure and were histopathologically diagnosed at Shanxian Central Hospital. For all samples, informed consent approved by the Independent Ethical Committees of Shanxian Central Hospital was obtained. Tissue samples were stored at −80°C.
Human melanoma cell lines, including MeWo, MHEM, A375, WM-115, and WM35, were purchased from the Chinese Academy of Sciences (Beijing, P.R. China) and were grown in RPMI-1640 medium (Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco). Primary human epidermal melanocytes (PEM; PromoCell, Beijing, P.R. China) from adult skin were maintained in serum- and PMA-free melanocyte growth medium M2 (PromoCell). All cells were cultured in a 37°C humidified atmosphere with 5% CO2.
Sun X., Zhang C., Cao Y, & Liu E. (2019). miR-150 Suppresses Tumor Growth in Melanoma Through Downregulation of MYB. Oncology Research, 27(3), 317-323.
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6

Melanoma Cell Lines and Dasatinib Preparation

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Lox-IMVI, Malme-3M, M14, Sk-Mel-5, and Sk-Mel-28 were obtained from the Department of Developmental Therapeutics, National Cancer Institute (NCI), HT144 from the American Tissue Culture Centre (ATCC) and WM-115 and WM-266-4 from the European Collection of Cell Cultures (ECACC). Lox-IMVI, Malme-3M, Sk-Mel-5, and Sk-Mel-28 were maintained at 37 °C with 5 % CO2 in RPMI medium with 10 % FCS (Cambrex). HT144 was maintained in McCoys 5A (Sigma-Aldrich) with 10 % FCS. WM-115 and WM-266- 4 were maintained in MEM media with 10 % FCS, 2 mM L-glutamine, 1 mM NEAA and 1 mM sodium pyruvate (all Gibco). Stock solutions of dasatinib (10 mM) (Sequoia Research Products) were prepared in dimethyl sulfoxide (Sigma-Aldrich).
Eustace A.J., Kennedy S., Larkin A.M., Mahgoub T., Tryfonopoulos D., O'Driscoll L., Clynes M., Crown J, & O'Donovan N. (2014). Predictive biomarkers for dasatinib treatment in melanoma. Oncoscience, 1(2), 158-166.
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7

Melanoma Cell Line Cultivation and Cytokine Treatment

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The human melanoma cell lines A375, wm115, and Sk-Mel-28 were obtained from American Type Culture Collection (Manassas, VA), and wm3211 was obtained from Rockland Immunochemicals (Limerick, PA). Cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; #11995073; Gibco, Waltham, MA) (A375) or Eagle’s Minimum Essential Medium (EMEM) (wm115, Sk-Mel-28) with 10% fetal bovine serum (FBS; #26140079; Gibco, Waltham, MA), or Tumor Specialized Media with 2% FBS (wm3211). Human immortal melanocyte cell lines (Hermes 1, 3a and 4a) were generously provided by Professor Dorothy C. Bennett (University of London, UK). The culture media and conditions followed the directions as provided by The Wellcome Trust Functional Genomics Cell Bank27 . Human IFN-γ was purchased from GoldBio (1160-06-100; St. Louis, MO) and IFN-α was obtained from PBL Assay Science (11100-1; Piscataway, NJ).
Tong S., Cinelli M.A., El-Sayed N.S., Huang H., Patel A., Silverman R.B, & Yang S. (2022). Inhibition of interferon-gamma-stimulated melanoma progression by targeting neuronal nitric oxide synthase (nNOS). Scientific Reports, 12, 1701.
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8

Malignant Melanoma Cell Lines TROAP Knockdown

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The human malignant melanoma cell lines A375 and WM-115 and human keratinocyte cells (HaCaT) utilized in this work were purchased from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (https://www.cellbank.org.cn). A375 and WM-115 cells were cultured in DMEM medium (Gibco BRL, United States) containing 10% fetal bovine serum (FBS), (Gibco BRL, United States), 1% streptomycin/penicillin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in 5% CO2. The cells were seeded in six-well plates and treated for 24 h with lentiviruses (Hanbio, Shanghai, China) including either shRNA sequences targeting TROAP or scrambled sequences (detailed lentivirus sequences for TROAP are available in Table S1) to construct TROAP knockdown and corresponding negative control cell lines respectively. Then, after 72 h of changing the medium, 1 μg/mL puromycin was added to kill uninfected cells. The efficiency of TROAP silencing in A375 and WM-115 cells was evaluated by real-time PCR analysis (RT-PCR).
Liu J., Pei S., Zhang P., Jiang K., Luo B., Hou Z., Yao G, & Tang J. (2023). Liquid-liquid phase separation throws novel insights into treatment strategies for skin cutaneous melanoma. BMC Cancer, 23, 388.
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