The following antibodies were used in this study: TEAD2 (21159-1-AP, Proteintech), actin (66009-1-Ig, Proteintech). The ferroptosis inducer erastin (B1524), ferroptosis inhibitor ferrostatin-1 (A4371), apoptosis inhibitor ZVAD-FMK (A1902) and necroptosis inhibitor necrostatin-1 (A4213) were obtained from APExBIO (Houston, USA).
Z vad fmk a1902
Manufactured by Apexbio
Sourced in United States
Z-VAD-FMK (A1902) is a broad-spectrum caspase inhibitor. It functions by irreversibly binding to the catalytic site of caspase enzymes, thereby inhibiting their activity.
Automatically generated - may contain errors
Lab products found in correlation
Actin 66009 1 ig, by Proteintech (1 mentions)
Ferrostatin 1 a4371, by Apexbio (1 mentions)
β actin 20536 1 ap, by Proteintech (1 mentions)
β catenin 8480, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 9661 t, by Cell Signaling Technology (1 mentions)
C myc 10828 1 ap, by Proteintech (1 mentions)
Fetal calf serum (fcs), by Sartorius (1 mentions)
4μ8c hy 19707, by MedChemExpress (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
3 protocols using z vad fmk a1902
1
Ferroptosis Induction and Inhibition
2
Cannabinoid Pathway Regulation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CBG (purity ≥98%, B20542) was purchased from Yuanye Biotechnology Co., LTD. (Shanghai, China), Z-VAD-FMK (A1902) and cycloheximide (CHX, A8244) were purchased from ApexBio (Houston, TX, USA), Suc-Leu-Leu-Val-Tyr-AMC (ab142120) and PML-RARA antibody (AB43152) were purchased from Abcam (Cambridge, MA, USA), lithium chloride (LiCl, L9650) was purchased from Sigma-Aldrich (St. Louis, MO, USA), cleaved caspase-3 (9661 T) and β-catenin (8480S) antibodies were purchased from Cell Signalling Technology (Danvers, MA, USA), microtubule-associated protein light chain 3 (LC3, 4600-1-AP), p62 (18420-1-AP), Bax (50599-2-Ig), Bcl-2 (12789-1-AP), Pro-caspase 3 (19677-1-AP), cyclin D1 (60186-1-Ig), c-myc (10828-1-AP), C/EBP homologous protein (CHOP, 60304-1-Ig), eukaryotic initiation factor 2α (eIF2α, 66482-1-Ig), protein kinase R-like endoplasmic reticulum kinase (PERK, 24390-1-AP), and β-actin (20536-1-AP) antibodies were purchased from Proteintech (Chicago, IL, USA), and p-eIF2α (Ser51) (AF3087), p-PERK (Thr982) (DF7576), and activating transcription factor 4 (ATF4, DF6008) antibodies were purchased from Affinity Biosciences (Cincinnati, OH, USA).
3
Anticancer Compounds Screening in Breast Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-231 and MCF7 cells were grown in Dulbecco's Modified Eagle's Medium (Gibco, NY, USA) containing 10% fetal calf serum (Biological Industries, Beit HaEmek, Israel). Glabridin (B20474) was purchased from Yuanye Bio-Technology (Shanghai, China). Paclitaxel (P106869), cycloheximide (C112766), and doxorubicin (D107159) were from Aladdin Biochemical Technology (Shanghai, China). GSK2656157 (HY13820) and 4μ8c (HY19707) were from Med-ChemExpress (NJ, USA). MG132 (GC10383) was purchased from Abcam (MA, USA) and Z-VAD-FMK (A1902) was from APExBIO (TX, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!