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Antibody to rabbit igg

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Antibody to rabbit IgG is a laboratory reagent used to detect and quantify rabbit immunoglobulin G (IgG) in various analytical applications. It functions as a secondary antibody that binds specifically to rabbit IgG, enabling its identification and measurement.

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Lab products found in correlation

Vinculin, by Merck Group (2 mentions) Her2 and her3, by Santa Cruz Biotechnology (2 mentions) Anti phosphotyrosine antibody clone 4g10 agarose conjugate, by Merck Group (2 mentions) Immunofluorescently labeled antibody to mouse igg, by Rockland Immunochemicals (2 mentions) Mk2206, by MedChemExpress (2 mentions) P mek, by Cell Signaling Technology (2 mentions) Odyssey scanner, by LI COR (2 mentions) Ripa buffer, by Merck Group (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Phosphatase inhibitor, by Roche (2 mentions) Gefitinib, by MedChemExpress (2 mentions)

Close spelling variants of this product

IgG Rabbit (4 citations)

2 protocols using antibody to rabbit igg

1

Molecular Signaling Analysis in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were homogenized in ice-cold RIPA buffer (Sigma) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor (Roche). For tumor lysates, standard western blotting was conducted as described 15 using antibodies against p-AKT (Ser473), p-AKT(Thr308), p-FoxO1 (Thr24)/FoxO3a(Thr32), p-ERK(1/2) (Thr202/Tyr 204), ERK, pMEK(Ser217/Ser221), MEK (Cell Signaling Technology), Her2 and Her3 (Santa Cruz Technology) and vinculin (Sigma-Aldrich). For immunoprecipitation assay, anti-phosphotyrosine antibody (clone 4G10) agarose conjugate (EMD Millipore) was used. Immunofluorescently labeled antibody to mouse IgG (Rockland Immunochemicals) and antibody to rabbit IgG (Molecular Probes) were used to visualize western blots on an Odyssey scanner (Li-Cor). MK2206 and Gefitinib were purchased from MedChemexpress and Selleck, respectively.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
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2

Molecular Signaling Analysis in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were homogenized in ice-cold RIPA buffer (Sigma) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor (Roche). For tumor lysates, standard western blotting was conducted as described 15 using antibodies against p-AKT (Ser473), p-AKT(Thr308), p-FoxO1 (Thr24)/FoxO3a(Thr32), p-ERK(1/2) (Thr202/Tyr 204), ERK, pMEK(Ser217/Ser221), MEK (Cell Signaling Technology), Her2 and Her3 (Santa Cruz Technology) and vinculin (Sigma-Aldrich). For immunoprecipitation assay, anti-phosphotyrosine antibody (clone 4G10) agarose conjugate (EMD Millipore) was used. Immunofluorescently labeled antibody to mouse IgG (Rockland Immunochemicals) and antibody to rabbit IgG (Molecular Probes) were used to visualize western blots on an Odyssey scanner (Li-Cor). MK2206 and Gefitinib were purchased from MedChemexpress and Selleck, respectively.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
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