The samples were redissolved in methanol and analyzed using an Applied Biosystems API2000 mass spectrometer equipped with an electrospray ionization (ESI) probe. The mobile phase was acetonitrile delivered at 50 μL/min, and the instrument parameters were as follows: curtain gas, 20 psi; ion spray voltage, 5500 V; nebulizer gas, 20 psi; declustering potential, 20 V; focusing potential, 200 V; entrance potential, 10 V. Product ion analyses were conducted with the collision gas set to 50 psi.
Api 2000 mass spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
The API 2000 mass spectrometer is an analytical instrument designed for the detection and identification of chemical compounds. It utilizes electrospray ionization technology to convert sample molecules into charged ions, which are then separated and detected based on their mass-to-charge ratio. The API 2000 provides sensitive and accurate measurements of molecular weights, allowing for the analysis of a wide range of substances.
Automatically generated - may contain errors
Lab products found in correlation
Am 300 spectrophotometer, by Bruker (1 mentions)
Bx 2 spectrometer, by PerkinElmer (1 mentions)
Melting point apparatus, by Gallenkamp (1 mentions)
Gemini nx, by Phenomenex (1 mentions)
Acetonitrile mecn, by Kanto Chemical (1 mentions)
Nh4cl, by Kanto Chemical (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Jnm ecs400, by JEOL (1 mentions)
Microtof q mass spectrometer, by Bruker (1 mentions)
1100 hplc system, by Thermo Fisher Scientific (1 mentions)
Avance drx 500 mhz, by Bruker (1 mentions)
Dionex ultimate 3000 hplc, by Thermo Fisher Scientific (1 mentions)
Avance 3 600 mhz, by Bruker (1 mentions)
6 protocols using api 2000 mass spectrometer
1
Mass Spectrometry Analysis of Methanol-Dissolved Samples
2
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were obtained from commercial sources and used without further purification, unless stated otherwise. Reaction progress was monitored by thin layer chromatography, using aluminum sheets precoated with silica gel 60 F254 (200 μm, Merck, Darmstadt, Germany). Melting points were determined on Weiss Gallenkamp melting point apparatus (Loughborough, England) and are uncorrected. IR spectra were recorded on Perkin Elmer BX-II spectrometer (Waltham, United States). 1H and 13C spectra were obtained using Bruker AM-300 spectrophotometer (Billerica, United States). Chemical shifts were described in parts per million. Mass spectra were recorded on an Applied Biosystems API 2000 mass spectrometer (Darmstadt, Germany).
3
Quantitative LC-MS/MS Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Shimadzu UFLCXR system coupled to an Applied Biosystems API2000 mass spectrometer was used. Column: Phenomenex Gemini-NX, 3 μm,110 Å, C18, 50 × 2 mm (at 40°C). Mobile phase: solvent A: 0.1% formic acid in water; solvent B: 0.1% formic acid in acetonitrile. Gradient: pre-equilibration for 1 min at 5% solvent B in solvent A; then linear gradient 5–98% solvent B over 2 min, 98% B for 2 min, 98–5% B over 0.5 min, then 5% B for 1 min. Flow rate: 0.5 mL/min. Detector: UV detection at 254 nm (channel 1), 220 nm (channel 2). Mass spectrometer: positive ion mode.
4
Quantification of Sarecycline and Minocycline in Plasma and Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The endpoint of this analysis was concentration of sarecycline or minocycline in plasma (μg/ml) and in brain samples (μg/g) at 1, 3, and 6 h postdosing. Plasma and brain homogenate samples were prepared by protein precipitation with acetonitrile, followed by centrifugation. The samples were injected on an API 2000 mass spectrometer (Applied Biosystems, Foster City, CA, USA) and analyzed in positive ion mode using doxycycline as an internal standard. Values were calculated using Analyst 1.2 quantitation software (SciEx, Framingham, MA, USA). Linear through zero regression analysis with no weighting factor was used to determine the calculated concentrations of the injected samples.
5
Synthesis and Characterization of Ir(III) Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Ir(III) complexes used in this study were synthesized and characterized according to the methods described in the Supplementary Information.
Acetonitrile (MeCN; Kanto Chemical, spectroscopic grade) and NH4Cl (Kanto Chemical, special grade) were used as received. Antimycin A from Streptomyces sp. (Ant A) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrozone (FCCP, 98%) were purchased from Sigma-Aldrich. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories.
1H-NMR spectra were recorded with a JEOL JNM-ECS400 (400 MHz) spectrometer in DMSO-d6. 1H-NMR chemical shifts were referenced to tetramethylsilane. The apparent resonance multiplicity was described as s (singlet), d (doublet), dd (double doublet), t (triplet), and m (multiplet). ESI–MS spectra were recorded on an Applied Biosystems API 2000 mass spectrometer.
Acetonitrile (MeCN; Kanto Chemical, spectroscopic grade) and NH4Cl (Kanto Chemical, special grade) were used as received. Antimycin A from Streptomyces sp. (Ant A) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrozone (FCCP, 98%) were purchased from Sigma-Aldrich. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories.
1H-NMR spectra were recorded with a JEOL JNM-ECS400 (400 MHz) spectrometer in DMSO-d6. 1H-NMR chemical shifts were referenced to tetramethylsilane. The apparent resonance multiplicity was described as s (singlet), d (doublet), dd (double doublet), t (triplet), and m (multiplet). ESI–MS spectra were recorded on an Applied Biosystems API 2000 mass spectrometer.
6
Comprehensive Analytical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melting points were determined on a Büchi 50 oil bath apparatus. Thin layer chromatography was performed using Merck aluminium sheets coated with silica gel 60 F 254 .
NMR spectra were recorded using Bruker Avance III-600 MHz and Bruker Avance DRX-500 MHz instruments. LC-DAD chromatograms and ESI-MS spectra were recorded on an Agilent 1100 HPLC system with an Applied Biosystems API-2000 mass spectrometer. HRMS was performed on a microTOF-Q mass spectrometer (Bruker, Köln, Germany) with ESI-source coupled with a HPLC Dionex Ultimate 3000 (Thermo Scientific, Braunschweig, Germany) using a EC50/2 Nucleodur C18 Gracity 3 µm column (Macherey-Nagel, Düren, Germany). A volume of one µL of a sample solution (1.0 mg/mL) was injected. Mobile phase was a mixture of 2 mM aqueous ammonium acetate solution and acetonitrile. Elution was performed from 90:10 up to 0:100 in 9 min, 0:100 for 5 min. Elemental analysis was performed with a vario MICRO
NMR spectra were recorded using Bruker Avance III-600 MHz and Bruker Avance DRX-500 MHz instruments. LC-DAD chromatograms and ESI-MS spectra were recorded on an Agilent 1100 HPLC system with an Applied Biosystems API-2000 mass spectrometer. HRMS was performed on a microTOF-Q mass spectrometer (Bruker, Köln, Germany) with ESI-source coupled with a HPLC Dionex Ultimate 3000 (Thermo Scientific, Braunschweig, Germany) using a EC50/2 Nucleodur C18 Gracity 3 µm column (Macherey-Nagel, Düren, Germany). A volume of one µL of a sample solution (1.0 mg/mL) was injected. Mobile phase was a mixture of 2 mM aqueous ammonium acetate solution and acetonitrile. Elution was performed from 90:10 up to 0:100 in 9 min, 0:100 for 5 min. Elemental analysis was performed with a vario MICRO
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!