CD8β (YTS156.7.7, Biolegend), CD45.2 (104, eBioscience), Epcam (G8.8, Biolegend), CD11b (M1/70, eBioscience), and CD11c (HL3, eBioscience). For identification of YFP-producing cells, sections were stained with anti-GFP (Abcam, ab6556), anti-rabbit-FITC (Abcam, ab6108), and anti-FITC-AlexaFlour488 (Invitrogen, polyclonal) antibodies. No reactivity was observed in infected YFP-negative mice. Stained slides were mounted with Prolong Gold antifade reagent (Thermo Fisher Scientific), imaged using a Nikon 90i, and analyzed using Adobe Photoshop software.
Cd45.2 104
The CD45.2 (104) is a laboratory instrument used for the detection and analysis of the CD45.2 antigen. It is a commonly used marker in immunology research and flow cytometry applications.
Lab products found in correlation
13 protocols using cd45.2 104
Multiparametric Analysis of Intestinal Immune Cells
Hematopoietic Stem Cell Transplantation Assay
Multiparametric T Cell Analysis
Laminin Isoforms and Cell Markers Analysis
Laminin 411 and 511 were purified as previously described (Sixt et al., 2001b (link)). Recombinant laminin α5 domain IVa was produced in HEK293 cells as described previously (Sasaki and Timpl, 2001 (link)); mini-laminin 511 composed of the C-terminal sequences of laminin α5, β1, and γ1 chains was from Takara Bio (Künneken et al., 2004 (link); Miyazaki et al., 2008 (link)).
Isolating Lung Immune Cells
Immunophenotyping of Mouse Leukocytes
Multiparameter Flow Cytometry Panel
Multiparameter Flow Cytometry of Immune Cells
Monoclonal Antibodies for Cell Identification
Flow Cytometry Immunophenotyping Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!