New plant genome extraction kit dp320

For further elucidating the expression pattern of GsAAE3, total DNA was isolated from the roots of Glycine soja (BW69) using the New Plant Genome Extraction Kit (DP320, TIANGEN, Beijing, China). The DNA sequence located 1819 bp upstream of the initiation site in GsAAE3 (Data S1B) was cloned into pCAMBIA3301 to generate the GsAAE3 promoter-GUS fusion plasmid under the control of CaMV 35S promoter. The GsAAE3 promoter-GUS fusion plasmid was introduced into soybean hairy roots via transformation with the Agrobacterium rhizogenes strain K599 [72 (link)]. After the culture in the nutrient solution added with 23 μM CdCl₂ or 25 μM AlCl₃ for 4 h, the soybean hairy roots were stained for GUS. The GUS activity of transformed roots was detected using the GUS Stain Kit (RTU4032, Real-Times). The corresponding primers used in the present study are listed in Table S2.

In this study, 29 tomato core collections were selected and cultivated in the experimental farm of Ningxia University (Table 1). The fresh and healthy leaves of 29 tomato germplasms were collected, and then the leaf tissue samples were frozen fresh at -80°C until DNA extraction. The total genomic DNA was extracted using the New Plant Genome Extraction Kit (DP320) (Tiangen, Beijing, China) according to the manufacturer’s instructions. The purity and integrity of the genomic DNA samples were identified using 1% agarose gel electrophoresis. The concentration of genomic DNA samples was measured using a NanoDrop 2000C spectrophotometer (Thermo Scientific; Waltham, MA, USA).