An enzymatic assay was used for determining the content of total cholesterol and free cholesterol levels of cells according to the manufacturer’s instructions (Applygen Technologies, E1015 and E1016, Beijing). Briefly, cells were washed with PBS to remove the remaining serum from the culture medium and then added with lysis buffer in the kit. The working solution and cholesterol standard substance were diluted. After incubation at 37 °C for 20 min, OD values were measured at 550 nm by multimode microplate reader (Thermo Fisher, USA) to calculate cholesterol content.
E1016
Manufactured by Applygen
Sourced in China
The E1016 is a laboratory equipment product designed for specific functions. It is a compact and versatile device, suitable for use in various research and testing environments. The core function of the E1016 is to perform essential tasks required in a laboratory setting. However, without further details about the product, a more specific description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using e1016
1
Enzymatic Cholesterol Quantification in Cells
2
Quantifying Cholesterol Fractions in Hypothalamus
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The contents of total cholesterol (Tch, E1015, Applygen Technologies, Inc. Beijing, China) and free cholesterol (E1016, Applygen Technologies, Inc. Beijing, China) in hypothalamus were determined according to the manufacturer's instructions. Cholesterol ester content was calculated by subtracting the free cholesterol content from the total Tch content.
3
Hepatic Cholesterol Quantification
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Hepatic total cholesterol and free cholesterol concentration were measured using a tissue total cholesterol assay kit (E1015; Applygen Technologies, Inc.) and free cholesterol assay kit (E1016; Applygen Technologies, Inc.) following the manufacturer's instructions. Cholesteryl ester was quantified by subtracting the free cholesterol values from the total cholesterol value.
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