Legendplex human anti virus response panel 13 plex

Manufactured by BioLegend

Sourced in United States

The LEGENDplex Human Anti-Virus Response Panel (13-plex) is a multiplex assay designed to measure the levels of 13 different analytes related to the human immune response against viruses. The panel allows for the simultaneous quantification of these analytes in a single sample.

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Lab products found in correlation

Bio bits cloud based software platform, by BioLegend (2 mentions) Legendplex human pro inflammatory chemokine panel 13 plex, by BioLegend (2 mentions) Anti cd86 pe, by BioLegend (1 mentions) Anti cd83 fitc, by BioLegend (1 mentions) Anti cd14 pb, by BioLegend (1 mentions) Anti mhc 2 fitc, by BioLegend (1 mentions) Facscanto 2, by BD (1 mentions) Legendplex data analysis software suite, by BioLegend (1 mentions)

Spelling variants of this product

LEGENDplex (292 citations) LEGENDplex Human Anti-Virus Response Panel (24 citations) Human Anti-Virus Response Panel (13-plex) (7 citations) LEGENDplex panels (5 citations) Legendplex Anti-virus response panel (4 citations) LEGENDplex™ Human Anti-Virus Response Panel 13-plex kit (2 citations)

7 protocols using legendplex human anti virus response panel 13 plex

Antiviral Cytokine Analysis of MoDCs

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At 24 h post-stimulation, supernatant and MoDCs were collected for analysis. Antiviral cytokines levels in the supernatant were measured using cytometric bead array [LEGENDplex™ Human Anti-Virus Response Panel (13-plex), BioLegend, San Diego, CA, USA].
Phenotypic maturation of MoDCs was analyzed by flow cytometry using anti-CD14 PB, anti-CD86 PE, anti-MHC II FITC, anti-CD83 FITC, and anti-CD155 APC (Biolegend, San Diego, CA, USA).

Luk A.D., Ni K., Wu Y., Lam K.T., Chan K.W., Lee P.P., Tu W., Mao H, & Lau Y.L. (2018). Type I and III Interferon Productions Are Impaired in X-Linked Agammaglobulinemia Patients Toward Poliovirus but Not Influenza Virus. Frontiers in Immunology, 9, 1826.

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Quantification of Antiviral Cytokines in Airway Samples

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Apical washes and basolateral medium were collected from infected HAE at indicated time points. Samples were subjected to the LEGENDplex Human Anti-Virus Response Panel (13-plex) (Biolegend, V-bottom plate cat# 740390). To describe the assay in brief, collected samples were incubated with specific capture-antibody-coated beads detecting 13 antiviral cytokines including IFN-α2, IFN-β, IFN-λ1, IFN-λ2/3, IFN-γ, IL-6, CXCL10, TNF-α, IL-8, IL-10, IL-12p70, GM-CSF, and IL-1β. Sample and beads were rocked at room temperature for 2 h. After the capture beads were washed, biotinylated antibodies were added and incubated with the capture beads for an additional hour. Lastly, phycoerythrin-conjugated streptavidin was added. Cytokine was quantified using flow cytometry and analyzed with the provided LEGENDplex analysis software (Qognit, Inc.).

Durnell L.A., Hippee C.E., Cattaneo R., Bartlett J.A., Singh B.K, & Sinn P.L. (2023). Interferon-independent processes constrain measles virus cell-to-cell spread in primary human airway epithelial cells. Microbiology Spectrum, 11(5), e01361-23.

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Multiplex Immunoassay for Cytokine Detection

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Open format DA-D4 slides were fabricated as described above using all reagents needed for antibody detection and IP-10 detection. Citrated plasma samples from 10 patients were procured from the ICU biorepository. 60 μL of each sample was added to two separate DA-D4 chips, incubated for 30 min, and the chips were then rinsed using 0.1% Tween in 1x PBS. All slides were scanned with the Genepix tabletop scanner.
IP-10 levels were measured using the LEGENDplex™ Human Proinflammatory Chemokine Panel (13-plex) and LEGENDplex™ Human Anti-Virus Response Panel (13-plex) obtained from BioLegend. Assays were performed with patient serum per the manufacturer’s instructions. The assay was performed using a Beckman Coulter CytoFLEX flow cytometer and data processing was performed using BioLegend’s Bio-Bits cloud-based software platform. Each sample was tested in triplicate, and the results are reported as mean of these triplicates.

Heggestad J.T., Kinnamon D.S., Olson L.B., Liu J., Kelly G., Wall S.A., Fontes C.M., Joh D.Y., Hucknall A.M., Pieper C., Naqvi I.A., Chen L., Que L.G., Oguin T I.I.I., Nair S.K., Sullenger B.A., Woods C.W., Sempowski G.D., Kraft B.D, & Chilkoti A. (2020). Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test. medRxiv.

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Quantifying Inflammatory Biomarkers in Plasma

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Open-format DA-D4 slides were fabricated as described above using all reagents needed for antibody detection and IP-10 detection. Citrated plasma samples from 10 patients were procured from the ICU biorepository. Sixty microliters of each sample was added to two separate DA-D4 chips and incubated for 30 min, and the chips were then rinsed using 0.1% Tween 20 in 1× PBS. All slides were scanned with the Genepix tabletop scanner.
IP-10 levels were measured using the LEGENDplex Human Proinflammatory Chemokine Panel (13-plex) and LEGENDplex Human Anti-Virus Response Panel (13-plex) obtained from BioLegend. Assays were performed with patient serum per the manufacturer’s instructions. The assay was performed using a Beckman Coulter CytoFLEX flow cytometer, and data processing was performed using BioLegend’s Bio-Bits cloud-based software platform. Each sample was tested in triplicate, and the results are reported as the mean of these triplicates.

Heggestad J.T., Kinnamon D.S., Olson L.B., Liu J., Kelly G., Wall S.A., Oshabaheebwa S., Quinn Z., Fontes C.M., Joh D.Y., Hucknall A.M., Pieper C., Anderson J.G., Naqvi I.A., Chen L., Que L.G., Oguin T I.I.I., Nair S.K., Sullenger B.A., Woods C.W., Burke T.W., Sempowski G.D., Kraft B.D, & Chilkoti A. (2021). Multiplexed, quantitative serological profiling of COVID-19 from blood by a point-of-care test. Science Advances, 7(26), eabg4901.

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Multiplex Cytokine Profiling of Vaccinated Participants

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Cytokines in serum obtained from vaccinated participants (n = 11) were quantified using a LEGENDplex Human Anti-Virus Response Panel (13-plex) (BioLegend, 740390) and a Human B cell Panel (13-plex) (BioLegend, 740527) according to the default protocols. In brief, the samples and standards were incubated with premixed beads on a plate shaker (800 rpm) for 2 hours at room temperature. After washing with 1× wash buffer, they were reacted with a detection antibody on a plate shaker (800 rpm) for 1 hour at room temperature, and then PE-conjugated beads were added and reacted on a plate shaker (800 rpm) for 30 minutes at room temperature. After washing with 1× wash buffer, the samples were read on a flow cytometer using a FACSCanto II (BD Biosciences). The Flow Cytometry Standard (FCS) files were analyzed using the LEGENDplex Data Analysis Software Suite (BioLegend), an online cloud-based program (https://legendplex.qognit.com/).

Yamaguchi Y., Kato Y., Edahiro R., Søndergaard J.N., Murakami T., Amiya S., Nameki S., Yoshimine Y., Morita T., Takeshima Y., Sakakibara S., Naito Y., Motooka D., Liu Y.C., Shirai Y., Okita Y., Fujimoto J., Hirata H., Takeda Y., Wing J.B., Okuzaki D., Okada Y, & Kumanogoh A. (2022). Consecutive BNT162b2 mRNA vaccination induces short-term epigenetic memory in innate immune cells. JCI Insight, 7(22), e163347.

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Monocyte Cytokine Response to R848 Stimulation

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After isolating monocytes from PBMCs collected before (D0) and after (D20 and D49) vaccination as described above, 1 × 10⁵ cells per well were cultured with RPMI 1640 (NACALAI TESQUE, 30264-85) and 100 ng/mL R848 (InvivoGen, tlrl-r848) in 96-well flat-bottom plates (Nunc) for 6 hours or 24 hours at 37°C and in 5% CO₂. After centrifugation at 300g for 5 minutes, the supernatant was transferred to another plate to perform a cytokine quantification assay, and the cells were resuspended in 350 μL of buffer RL containing 143 mM 2-mercaptoethanol (NIPPON Genetics Co. Ltd., FG-81250) for RNA extraction and subsequent qPCR. These samples were stored at −80°C until use. Cytokines in the supernatant were quantified using a LEGENDplex Human Anti-Virus Response Panel (13-plex) (BioLegend, 740390) as described above for serum cytokine quantification.

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Cytokine and IFN-γ Quantification

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Cytokine quantification of plasma samples was performed with LEGENDplex™ Human Anti-Virus Response Panel (13-plex) (ref. 740390, Biolegend, CA, USA), according to manufacturer’s instructions. IFN-γ produced upon peptide stimulation was quantified in cell culture supernatant using an ELISA MAX Human IFN-γ (ref. 430104, BioLegend, CA, USA), according to the manufacturer’s instructions.

Santa Cruz A., Mendes-Frias A., Azarias-da-Silva M., André S., Oliveira A.I., Pires O., Mendes M., Oliveira B., Braga M., Lopes J.R., Domingues R., Costa R., Silva L.N., Matos A.R., Ângela C., Costa P., Carvalho A., Capela C., Pedrosa J., Castro A.G., Estaquier J, & Silvestre R. (2023). Post-acute sequelae of COVID-19 is characterized by diminished peripheral CD8+β7 integrin+ T cells and anti-SARS-CoV-2 IgA response. Nature Communications, 14, 1772.

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