Klenow fragment (KF-) polymerase (without 3′ to 5′ exonuclease activity) and 10x New England Biolabs (NEB) buffer 2 (100 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl2, 10 mM DTT, pH = 7.9) were obtained from New England Biolabs (Beverly, MA, USA). DNA probe (5'-FAM-CCCCCCCCCGTT CTTCCCTTGTTCG-3') was synthesized by Sangon Biotechnology Co. Ltd. (Shanghai, China). dNTP mixture and TE buffer were purchased from Sangon Biological Engineering Technology and Services Co., Ltd. (Shanghai, China). The Mercury chloride (HgCl2) and silver nitrate (AgNO3) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The potassium chloride (KCl), lithium nitrate (LiNO3), calcium(II) nitrate tetrahydrate (Ca(NO3)2∙4H2O), copper(II) nitrate trihydrate [CuNO3)2∙3H2O], manganese(II) nitrate tetrahydrate [Mn(NO3) 4H2O], iron(III) nitrate nonahydrate (Fe(NO3)3∙9H2O), zinc chloride (ZnCl2) and iron(II) chloride tetrahydrate (FeCl2∙4H2O) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Ultra-pure water (18 MΩ∙cm-1, Kertone Ltd., Changsha, China) was used during all the experiments. The DNA probe was dissolved in TE buffer to 100 μM and stored at −20 °C for further use.
Copper 2 nitrate trihydrate
Manufactured by Sinopharm
Sourced in China
Copper(II) nitrate trihydrate is a chemical compound with the formula Cu(NO3)2·3H2O. It is a blue crystalline solid that is soluble in water. The compound is commonly used as a precursor for other copper-containing compounds and in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
N n dimethylformamide (dmf), by Sinopharm (3 mentions)
Zinc 2 nitrate hexahydrate, by Sinopharm (2 mentions)
Ethanol, by Sinopharm (2 mentions)
Manganese 2 nitrate tetrahydrate, by Sinopharm (1 mentions)
Dna probe, by Sangon (1 mentions)
Mercury chloride hgcl2, by Merck Group (1 mentions)
Silver nitrate agno3, by Merck Group (1 mentions)
Iron 3 nitrate nonahydrate, by Sinopharm (1 mentions)
Polyvinylpyrrolidone, by J&K Scientific (1 mentions)
Trimesic acid, by J&K Scientific (1 mentions)
Anhydrous ferric chloride, by Sinopharm (1 mentions)
Hydrochloric acid (hcl), by Sinopharm (1 mentions)
Tetrakis triphenylphosphine palladium, by Merck Group (1 mentions)
Nickel 2 nitrate hexahydrate, by Sinopharm (1 mentions)
Gold trichloride, by Merck Group (1 mentions)
Magnesium 2 nitrate hexahydrate, by Sinopharm (1 mentions)
Potassium carbonate, by Sinopharm (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Iron 3 chloride hexahydrate, by Sinopharm (1 mentions)
Sodium carbonate, by Sinopharm (1 mentions)
8 protocols using copper 2 nitrate trihydrate
1
Metal Ion-Dependent DNA Detection
2
Synthesis of Sulfur-Containing Heterocyclic Compounds
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Benzothiophene (BT, 98%) and dibenzothiophene (DBT, 98%) were purchased from Shanghai D&B Chemical Technology Co., Ltd., China. Copper (II) nitrate trihydrate [Cu(NO3)2·3H2O, 99%] and tetrabutylortotitanate (TBOT, 99%) were supplied by Sinopharm Chemical Reagent Co., Ltd., Beijing, China. Trimesic acid [C6H3(COOH)3, 99%] and polyvinyl pyrrolidone (PVP, 99%, Mr 40000) were provided by J&K Scientific Ltd., Shanghai, China. All other reagents were commercially available and used without further purification.
3
Textile Waste Valorization using Metal Salts
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The WPTs used in this research were recovered from a textile mill in Nantong, Jiangsu Province, China. The primary chemicals in this research were zinc chloride (ZnCl2), anhydrous ferric chloride (FeCl3), copper(II) nitrate trihydrate (Cu(NO3)2·3H2O), and hydrochloric acid (HCl), which were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). Analytical pure−grade chemical reagents were used for all experiments, and ultrapure water was used to prepare the solutions for the experiments.
4
Synthesis of Metal-Organic Frameworks
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All chemicals were used without further purification unless otherwise specified. p-Phenylenediamine, 4-bromobenzonitrile, and tert-butyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole-1-carboxylate were supplied by Energy Chemical. Gold trichloride and tetrakis(triphenylphosphine)palladium were bought from Sigma-Aldrich. 3,3′,5,5′-Azobenzene tetra carboxylic acid, trimesic acid, and terephthalic acid were purchased from Acmec. Sodium hydroxide, iron(III) chloride hexahydrate (97%), copper(II) nitrate trihydrate (99%), nickel(II) nitrate hexahydrate (99%), sodium nitrate (99%), magnesium(II) nitrate hexahydrate (99%), potassium nitrate, zinc(II) nitrate hexahydrate (99%), potassium carbonate, sodium carbonate, sodium sulfate, and all solvents were provided by Sinopharm Chemical Reagent Co. Ltd. The Yangtze River water was collected in Nanjing, China (latitude: 32.156613, longitude: 118.800723). The East China Sea water was collected in Xiamen, China (latitude: 24.442217, longitude: 118.075667).
5
Synthesis of Copper Nitrate Hydrate
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ND powders were purchased from Beijing Grish Hitech Co., Ltd, China. Copper (II) nitrate trihydrate (Cu(NO3)2·3H2O) was the analytical reagent and purchased from Sinopharm Chemical Reagent Co., Ltd, China. Deionized (DI) water with the specific resistance of 18.25 MΩ cm was used in all our experiments.
6
Synthesis of Metal-Organic Frameworks
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All reagents and solvents were commercially available, which were directly used without purification, unless otherwise indicated. Zinc(II) nitrate hexahydrate (Zn(NO3)2·6H2O) (99%), copper(II) nitrate trihydrate (Cu(NO3)2·3H2O) (99%), acetone (≥99.5%), isopropanol (≥99.7%), methanol (≥99.5%), ethanol (≥99.7%), triethylamine (TEA) (≥99.5%), toluene (≥99.5%), N,N-dimethylformamide (DMF) (≥99.5%), ammonium hydroxide (NH3·H2O) (25–28%), and acetic acid (HAc) (≥99.8%) were purchased from Sinopharm. 1,3,5-Benzenetricarboxylic acid (H3BTC) (98%), styrene (99.5%), phenylacetylene (98%), and 2-methylimidazole (98%) were obtained from Energy Chemical.
7
Synthesis of Copper-Porphyrin Complex
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Copper(II) nitrate trihydrate (Cu(NO3)2·3H2O) and DMF were bought in Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Tetrakis(4-carboxyphenyl)porphyrin (H2TCPP), Hydroquinone (HQ), and Catechol (CC) were purchased from Aladdin Chemistry Co., Ltd. (Shanghai, China). All chemicals were used without further purification.
8
Copper(II) Nitrate-based Electrochemical Sensor
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Copper(II) nitrate trihydrate (Cu(NO3)2·3H2O), graphite powder (SP), N,N-dimethylformamide (DMF), N-methyl-2-pyrrolidone (NMP), and ethanol were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Tetrakis(4-carboxyphenyl)porphyrin (H2TCPP) and dopamine hydrochloride were purchased from Aladdin Chemistry Co., Ltd. (Shanghai, China). High-purity water was used for all the experiments.
The structural properties of the prepared materials were characterized using X-ray diffraction pattern (XRD, X’Pert PRO diffractometer, Cu kα1 radiation, Panalytical Company, Almelo, The Netherlands) and Fourier transform infrared spectroscopy (FT-IR, Equinox-55 Fourier transform infrared spectrometer, Bruker, Karlsruhe, Germany). The morphological properties were studied using scanning electron microscopy (Nova NanoSEM 450 microscope, FEI Company, Eindhoven, The Netherlands) and transmission electron microscopy (Tecnai G2 F30 microscope, FEI Company, Eindhoven, The Netherlands).
Electrochemical tests were performed on a CHI 660E electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd., Shanghai, China) using a conventional three-electrode system. A Cu-TCPP/graphene/GCE (diameter: 3 mm) as the working electrode, a saturated calomel electrode (SCE) as the reference electrode, and a platinum wire as the counter electrode were used.
The structural properties of the prepared materials were characterized using X-ray diffraction pattern (XRD, X’Pert PRO diffractometer, Cu kα1 radiation, Panalytical Company, Almelo, The Netherlands) and Fourier transform infrared spectroscopy (FT-IR, Equinox-55 Fourier transform infrared spectrometer, Bruker, Karlsruhe, Germany). The morphological properties were studied using scanning electron microscopy (Nova NanoSEM 450 microscope, FEI Company, Eindhoven, The Netherlands) and transmission electron microscopy (Tecnai G2 F30 microscope, FEI Company, Eindhoven, The Netherlands).
Electrochemical tests were performed on a CHI 660E electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd., Shanghai, China) using a conventional three-electrode system. A Cu-TCPP/graphene/GCE (diameter: 3 mm) as the working electrode, a saturated calomel electrode (SCE) as the reference electrode, and a platinum wire as the counter electrode were used.
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