Vitek 2 gram negative identification card

Manufactured by bioMérieux

Sourced in France

The VITEK 2 Gram-negative identification card is a lab equipment product used for the identification of Gram-negative bacteria. It provides a standardized, automated process for the identification of a wide range of Gram-negative microorganisms.

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Lab products found in correlation

Vitek 2 compact system, by bioMérieux (2 mentions) Gelcompar, by bioMérieux (1 mentions) Vitek 2 gn id, by bioMérieux (1 mentions) Vitek 2 system, by bioMérieux (1 mentions) E coli atcc 25922, by American Type Culture Collection (1 mentions) P aeruginosa atcc 27853, by American Type Culture Collection (1 mentions) K pneumoniae atcc 2146, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

VITEK 2 Gram-Negative Identification (GN) cards Vitek 2

4 protocols using vitek 2 gram negative identification card

Epidemiology of E. coli Bloodstream Infections

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E. coli, representing about14.6%(131/898), 18.0%(87/482) and 29.6%(56/189) of all the BSIs episodes in HA, HB and HC respectively, was the most frequent bacilli from BSI in each hospital. A total of 145 E. coli isolates (58 from HA, 31 from HB, 56 from HC) obtained from blood between 1 Sept 2013 and 30 Sept 2014 were collected. Ninety isolates were enrolled: thirty isolates were selected at random from each hospital using the random number generation function in Microsoft Office Excel2010 (Microsoft Corporation, Redmond, WA, USA). Only nonrepeat isolates from true incident cases were included. All isolates were identified by the VITEK 2 compact system and VITEK 2 Gram-negative identification card (bioMérieux, Marcyl’Étoile, France) and stored at -80°C in broth containing 30% glycerol until used.
This study was approved by Ruijin Hospital Ethics Committee (Shanghai Jiao Tong University School of Medicine), and the Review Board exempted request for informed consent because this retrospective study only focused on the bacteria and did not affect the patients.

Wang S., Zhao S.Y., Xiao S.Z., Gu F.F., Liu Q.Z., Tang J., Guo X.K., Ni Y.X, & Han L.Z. (2016). Antimicrobial Resistance and Molecular Epidemiology of Escherichia coli Causing Bloodstream Infections in Three Hospitals in Shanghai, China. PLoS ONE, 11(1), e0147740.

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Bacterial Identification and Antimicrobial Susceptibility

Cited 1 time

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Clinical samples — blood, sputum, throat swab, midstream urine, hydrothorax, and ascites — were collected by conventional methods recommended in the clinic. Samples were processed according to the standard operating procedure of our institution. All isolates were identified by the VITEK 2 compact system and VITEK 2 Gram-negative identification card (BioMérieux, Marcy-l’Étoile, France). The microbiology laboratory performed antimicrobial-susceptibility testing of the Gram-negative bacterial isolates based on minimum inhibitory concentration. More detailed methods have been described in our previous studies.13 (link),14 (link)

Zhang Y., Zheng Y., Dong F., Ma H., Zhu L., Shi D., Li X., Li J, & Hu J. (2020). Epidemiology of Febrile Neutropenia Episodes with Gram-Negative Bacteria Infection in Patients Who Have Undergone Chemotherapy for Hematologic Malignancies: A Retrospective Study of 10 Years' Data from a Single Center. Infection and Drug Resistance, 13, 903-910.

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Identification and Antimicrobial Resistance Profiling of Bacterial Isolates

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We performed routine identification using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and biochemical species identification. For MALDI-TOF mass spectrometry, we used Biotyper MBT Smart (Bruker Corporation, https://www.bruker.com) with flexControl and MBT Compass version 4.1 software. We considered scores >2.0 high confidence identification and scores of 1.7–2.0 low confidence identification. We used VITEK 2 gram negative identification card (bioMérieux, https://www.biomerieux.com) for biochemical species identification. Phenotypic antimicrobial resistance profiles were determined using disk diffusion. We interpreted breakpoints according to Clinical and Laboratory Standards Institute (9 ) standards for Bcc (ceftazidime, trimethoprim/sulfamethoxazole, and meropenem) or Enterobacteriaceae (aminoglycosides, ciprofloxacin, piperacillin/tazobactam, and other β-lactams). We used XbaI to digest DNA before using previously described pulsed-field gel electrophoresis (PFGE) molecular typing principles (10 (link)). We used GelCompar (Applied Maths, http://www.applied-maths.com) to analyze PFGE results.

Seth-Smith H.M., Casanova C., Sommerstein R., Meinel D.M., Abdelbary M.M., Blanc D.S., Droz S., Führer U., Lienhard R., Lang C., Dubuis O., Schlegel M., Widmer A., Keller P.M., Marschall J, & Egli A. (2019). Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland. Emerging Infectious Diseases, 25(6), 1084-1092.

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Antibiotic Resistance in Colombian Isolates

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A total of 60 clinical strains were tested in this study: 30 each for K. pneumoniae and P. aeruginosa. All clinical isolates were isolated from clinical specimens, such as urine, secretions, and blood, recovered from two tertiary care hospitals in Cali, Colombia, between 2017 and 2019. All cultures of clinical isolates were sent to Microbiology Laboratory at Laboratorio de Salud Pública Departamental del Valle del Cauca (LSPD-Valle), where bacterial identity was confirmed, and antibiotic susceptibility characterization was performed. Species identification was performed using the automated VITEK^® 2 system, (bioMerieux, 9.02, Marcy l’Etoile, France) with the VITEK^® 2 Gram-Negative Identification card (VITEK^® 2 GN ID), which is based on established biochemical methods and substrates that evaluate the use of carbon, enzymatic activity, and resistance (Ref. 21341, bioMerieux, Marcy l’Etoile, France). All laboratory strains, including E. coli ATCC^® 25922™, K. pneumoniae ATCC^® 2146™, and P. aeruginosa ATCC^® 27853™, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Rivera-Sánchez S.P., Agudelo-Góngora H.A., Oñate-Garzón J., Flórez-Elvira L.J., Correa A., Londoño P.A., Londoño-Mosquera J.D., Aragón-Muriel A., Polo-Cerón D, & Ocampo-Ibáñez I.D. (2020). Antibacterial Activity of a Cationic Antimicrobial Peptide against Multidrug-Resistant Gram-Negative Clinical Isolates and Their Potential Molecular Targets. Molecules, 25(21), 5035.

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