Blood was drawn from the postcava and centrifuged at 3,000 × g for 5 min. Serum levels of AST and ALT were determined by an automated chemical analyzer (Hitachi Co, Tokyo, Japan). For liver histopathology, liver tissues were taken from the left lobe after 6 h reperfusion and were fixed in 4.5% buffered formalin. Paraffin embedding was performed using standard techniques. Biopsies were then sectioned and stained with Hematoxylin-Eosin and were examined under light microscope for histological changes. Liver histologic injury was assessed using a semi-quantitative light microscopy evaluation29 (link). Hepatic injury was demonstrated by the total score from each parameter. The presence of neutrophils in liver was assessed by myeloperoxidase (MPO) staining as previously described8 (link).
Automated chemical analyzer
Manufactured by Hitachi
Sourced in Japan
The Automated Chemical Analyzer is a laboratory instrument designed to perform quantitative analysis of chemical compounds. It automates the process of sample preparation, reagent addition, and measurement, increasing efficiency and precision in analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using automated chemical analyzer
1
Liver Injury Histopathology and Biomarkers
2
Liver Injury Assessment after CLP
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To assess hepatocellular injury following CLP, serum alanine transaminase (ALT) and aspartate aminotransferase (AST) were measured using an Automated Chemical Analyzer (Hitachi Co., Tokyo, Japan).
3
Serum Enzyme Measurement Protocol
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Blood was drawn from the inferior vena cava and centrifuged at 3,000 g for 5 min. The serum levels of AST and ALT were determined using an automated chemical analyzer (Hitachi Co., Tokyo, Japan).
4
Hepatocellular Damage Assessment Methods
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Hepatocellular damage was assessed in vivo by measuring serum AST and ALT levels using an automated chemical analyzer (Hitachi Co, Tokyo, Japan), and in vitro by measuring medium LDH using a colorimetric LDH cytotoxicity assay kit (BioVision, Milpitas, CA) according to the manufacturer's protocol.
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