Dp30bw cd camera

Fluorescence microscopy and Nomarski optics microscopy were done using an Olympus BX60 microscope equipped with DP30BW CD camera (Olympus, Tokyo, Japan).

Fluorescence microscopy and Nomarski optics microscopy were done using an Olympus BX60 microscope equipped with DP30BW CD camera (Olympus, Tokyo, Japan). Animals were analyzed on microscopic glass slides with a thin layer of 2% agarose and immobilized by 1 mM levamisole (Sigma-Aldrich, St. Louis, MO, USA). Confocal microscopy of live homozygous animals with edited F28F8.5 expressing gfp::F28F8.5 was performed using an inverted Leica SP8 TCS SMD FLIM system equipped with a 63 × 1.2 NA water immersion objective, a pulsed white light laser (470–670 nm), AOBS and two internal hybrid single photon counting detectors, and operated by Leica Application Suite X program (Leica Microsystems, Wetzlar, Germany). The GFP fluorescence was excited at a wavelength of 488 nm and the emitted light was simultaneously recorded in two spectral ranges (Channel 1—495 nm to 525 nm, Channel 2—525 nm to 580 nm; the two channel setup was used to help resolve between spectrally different autofluorescence and GFP fluorescence signals).