Carbonic anhydrase

The library compounds used in this study are all FDA-approved drugs. For all the experiments conducted with 176 compounds, the drug molecules are selected from the Prestwick library, which was obtained from the High Throughput Screening Laboratory at the University of Kansas. The library was provided in combined batches of 176 compounds per vial, dissolved in DMSO at a concentration of 14.2 μM per compound. For all the experiments conducted with 352 compounds, the drug molecules were obtained from LOPAC1280-small scale library that was purchased from Sigma Aldrich (St. Louis, MO) as individual compounds, dissolved in DMSO at a concentration of 10 mM per compound. When combining compounds for high throughput screens from the LOPAC library, any compound larger than 150 Da was considered a candidate, and no effort was made to remove compounds from the library that would likely not ionize by ESI. Pepsin, Aminolink plus coupling resin and disposable plastic columns were acquired from Pierce Biotechnology, Inc (Rockford, IL), while nitrocellulose drop dialysis membranes were purchased from Fisher Scientific (Houston, TX). Maltose binding protein and Carbonic Anhydrase were purchased from My BioSource (San Diego, CA) and Sigmal Aldrich (Milwaukee, WI), respectively.