Denaturing buffer 10

Chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless specified otherwise. Aminolink resin and SCSC were purchased from Life Technologies (Grand Island, NY). Alltech extract clean Carbograph were from Grace (Columbia, MD). Hypercarb AutoTip (TT2CAR; 10–200 μL) was packed by Glygen Corporation (Columbia, MD). C18 analytical LC column (NanoViper, 75 μm, 150 mm, 2 μm particle size) were from Fisher Scientific (Waltham, MA). Eppendorf 96-well plate, matrix D.A.R.T.s tips (20–300 μL), automation reservoirs were purchased from Thermo Fisher Scientific (Hudson, NH). Denaturing buffer (10×), GlycoBuffer (10×), and Peptide-N-glycosidase F (PNGase F) were from New England Biolabs (Ipswich, MA). Trypsin gold (mass spectrometry (MS) grade) were ordered from Promega Corporation (Madison, WI). The polyethylene sheet has typical median pore size 15–45 μm (thickness = 1.57 mm or 0.062 in; Interstate Specialty Products; Sutton, MA).

All chemicals and reagents were purchased from Sigma Aldrich (St Louis, MO) unless specified otherwise. C18 Solid-phase extraction (SPE) cartridges (3 cc Vac Cartridge, 500 mg sorbent) were from Waters Corporation (Milford, MA). Peptide-N-glycosidase F (PNGase F) and denaturing buffer (10×) were from New England Biolabs (Ipswich, MA). Trypsin gold was from Promega (Madison, WI). Quantitative analysis of peptides was performed on a Q-Exactive mass spectrometer (Thermo Scientific, Waltham, MA). Human lung tissues were collected from the Department of Pathology with the approval of the Institutional Review Board of the Johns Hopkins University.