Horseradish peroxidase conjugated anti mouse 7074p2

AGS cells were purchased from American Type Culture Collection (ATCC, Manassas, VA). MKN45 cells were obtained from the Riken Cell Bank (Tsukuba, Japan). AGS cells were cultured in F12 media (GIBCO, Carlsbad, CA) and MKN45 cells were cultured in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% fetal bovine serum (FBS, Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). All cell lines were ascertained to conform to the original in vitro morphological characteristics and were authenticated using short tandem repeat (STR) profiling (Genetica DNA Laboratories, Burlington, NC). All cell lines reported here have been tested and had shown to be free of mycoplasma (R&D Systems, Minneapolis, MN). AKT (9272s), p-AKT (S473, 4060s), p-STAT3 (Y705, 9193s), STAT3 (4904s), β-actin (4970) and horseradish peroxidase-conjugated anti-mouse (7074P2), and anti-rabbit (7062P2) secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA). DARPP-32 (sc-398144) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Trichostatin A and 5-Aza-2′-deoxycytidine were purchased from Sigma-Aldrich (St. Louis, MO). Cis-platin and Gefitinib (ZD1839) were purchased from Selleck Chemicals (Houston, TX) and MiR490–3p mimic and inhibitor were purchased from Applied Biological Materials (Richmond, BC, Canada).

AGS cells were obtained from American Type Culture Collection (ATCC, Manassas, VA). MKN-45 cells were purchased from the Riken Cell Bank (Tsukuba, Japan). AGS cells were cultured in F12 media (GIBCO, Carlsbad, CA) supplemented with 5% fetal bovine serum (FBS, Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). MKN-45 were cultured in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% FBS and 1% penicillin/streptomycin. All cell lines were authenticated using short tandem repeat (STR) profiling (Genetica DNA Laboratories, Burlington, NC) to conform to the original in vitro morphological characteristics. JAK2 (3230s), p-JAK2 (3776s), p-STAT3 (Y705, 9193s), STAT3 (4904s), p-SRC (Y416, 6943s), SRC (2109), p-IGF1R (Y1135/1136, 3024s), IGF1R (9750s), β-actin (4970) and horseradish peroxidase-conjugated anti-mouse (7074P2) and anti-rabbit (7062P2) secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA). GP130 (sc-655), p-GP130 (sc-22346), and DARPP-32 (sc-398144) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant Human IL-6 was purchased from PeproTech (Rocky Hill, NJ). Dasatinib and OSI-096 were purchased from Selleck Chemicals (Houston, TX).