Photographs of in vitro cultures and histological staining were taken with a stereomicroscope (Stemi 2000-C) equipped with a Zeiss CL 6000 LED illumination unit, and a video adapter 60 C including an AxioCam ERc 5s digital camera (all from Carl Zeiss MicroImaging). Trichome visualization through polarized light microscopy was carried out as described previously (Gudesblat et al., 2012 (link); Pomeranz et al., 2013 (link)) with the only change that destaining was achieved with a saturated chloral hydrate solution (dissolve 1 kg in 400 mL of water). Trichomes were visualized using a Nikon AZ100 zoom microscope equipped with a Nikon DS-Ri2 color camera. Microscopy images of trichomes and DAPI-stained nuclei were obtained using the AxioImager system (Zeiss) equipped with two cameras (AxioCam MRm and AxioCam MRc5).
Az100 zoom microscope
Manufactured by Nikon
The Nikon AZ100 zoom microscope is a versatile laboratory equipment designed for a wide range of imaging and analysis applications. It features a zoom range that allows for smooth and continuous magnification adjustment, enabling users to observe samples at various magnification levels. The AZ100 is equipped with advanced optics and illumination systems to deliver high-quality, detailed images. Its core function is to provide users with the ability to closely examine and analyze specimens under precise magnification control.
Automatically generated - may contain errors
Lab products found in correlation
Axiocam mrm, by Zeiss (1 mentions)
Axioimager system, by Zeiss (1 mentions)
Axiocam erc5s digital camera, by Zeiss (1 mentions)
Ds ri2 color camera, by Nikon (1 mentions)
Axiocam mrc5, by Zeiss (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Seaplaque agarose, by Lonza (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
2 protocols using az100 zoom microscope
1
Visualizing Plant Trichome Structures
2
Anchorage-Independent Cell Growth Assay
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Twelve-well plates were coated with 0.8% agar (low melting SeaPlaque Agarose, Lonza) in DMEM supplemented with 10% FBS (Gibco, Thermo Fisher Scientific) and 1× penicillin/streptomycin (Gibco, Thermo Fisher Scientific). On top, 1,500 cells per well in 0.4% agar (low melting SeaPlaque Agarose, Lonza) in DMEM with 10% FBS and 1× penicillin/streptomycin were seeded and air dried for 45 minutes at room temperature. After incubation at 37°C and 5% CO2 for 24 hours, 500 μL medium was added. Medium was changed twice a week and after 14–16 days, colonies were stained with 500 μL 0.005% Crystal Violet (Sigma, Merck) for 1 hour. Then destaining by washing three times with PBS was performed and pictures of colonies were taken on a AZ100 Zoom Microscope (Nikon).
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