Human and mouse samples were fixed in formalin, dehydrated, embedded in paraffin, and sectioned. Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) was used according to manufacturer’s instruction. Regarding human studies in Fig. 4 , skin samples from 5 healthy individuals and 5 patients with each following disease or disease subtype were used: dcSSc with an anti-topoisomerase I antibody, limited cutaneous SSc with an anti-centromere antibody, dcSSc with an anti-RNA polymerase III antibody, morphea, and eosinophilic fasciitis. As for mouse studies, the back skin and the right lower lobe of the lung were harvested. Skin thickness and cell counts were scored by S.N. and Y.A. in at least randomly chosen 5 high power fields of samples and the average number for each sample was used. Each section was examined independently in a blinded fashion. FITC-conjugated anti-rabbit (sc-2090, Santa Cruz) and Alexa Fluor 555-conjugated anti-goat (A21432; Life technologies, Carlsbad, CA, USA) were used for immunofluorescence studies as secondary antibodies at a 1/200 dilution.
Fitc conjugated anti rabbit sc 2090
Manufactured by Santa Cruz Biotechnology
Sourced in United States
FITC-conjugated anti-rabbit (sc-2090) is a secondary antibody conjugated with the fluorescent dye FITC (fluorescein isothiocyanate). Its core function is to detect and visualize rabbit primary antibodies in various immunological techniques, such as immunofluorescence, flow cytometry, and Western blotting.
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Lab products found in correlation
2 protocols using fitc conjugated anti rabbit sc 2090
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Immunohistochemical Analysis of Skin and Lung
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Immunohistochemical Analysis of Skin and Lung
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Human and mouse samples were fixed in formalin, dehydrated, embedded in paraffin, and sectioned. Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) was used according to manufacturer’s instruction. Regarding human studies in Fig. 4 , skin samples from 5 healthy individuals and 5 patients with each following disease or disease subtype were used: dcSSc with an anti-topoisomerase I antibody, limited cutaneous SSc with an anti-centromere antibody, dcSSc with an anti-RNA polymerase III antibody, morphea, and eosinophilic fasciitis. As for mouse studies, the back skin and the right lower lobe of the lung were harvested. Skin thickness and cell counts were scored by S.N. and Y.A. in at least randomly chosen 5 high power fields of samples and the average number for each sample was used. Each section was examined independently in a blinded fashion. FITC-conjugated anti-rabbit (sc-2090, Santa Cruz) and Alexa Fluor 555-conjugated anti-goat (A21432; Life technologies, Carlsbad, CA, USA) were used for immunofluorescence studies as secondary antibodies at a 1/200 dilution.
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