Whole blood samples in potassium‐EDTA tubes for CBC (Advia 120 or 2120i, Siemens, Medical Solutions Diagnostics GmbH, Erlangen,, Germany; Abacus Junior Vet, Diatron, Wien, Austria) and whole blood samples in plain tubes with gel separators for serum biochemistry (Cobas Integra 400 Plus or Cobas 6000, Roche, Mannheim, Germany) were collected at presentation and analyzed within 60 minutes after collection. Urine samples were obtained by cystocentesis for urinalysis including dipstick chemistry (Urilux, Roche, Mannheim Germany) and sediment cytology (SediVue Dx, IDEXX Laboratories Inc, Westbrook, Maine or manual microscopy), as well as for bacterial culture. Urine specific gravity (USG) was measured using a clinical refractometer (Atago, Tokyo, Japan). Pyuria and hematuria were defined as presence of >5 leukocytes or erythrocytes, respectively per high‐power field. Bacteriuria was diagnosed if bacteria were observed in sediment cytology. Proteinuria was defined as a urine dipstick result of ≥1+ (ie, ≥30 mg/dL). Urine protein‐to‐creatinine ratio (Cobas Integra 400 Plus or Cobas 6000, Roche, Mannheim, Germany) was only measured in dogs with severe proteinuria (urine dipstick result +4) and clinical concern that glomerular disease was the inciting cause for ACKD.
Sedivue dx
Manufactured by IDEXX
Sourced in Germany
The SediVue Dx is a laboratory equipment product designed to automate the analysis of urine sediment samples. It uses digital imaging technology to capture and analyze microscopic elements in the urine, providing objective, standardized results.
Automatically generated - may contain errors
5 protocols using sedivue dx
1
Comprehensive Diagnostic Evaluation of CKCD
2
Comprehensive Canine Health Evaluation
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Blood samples for CBC (Advia 120 or 2120, Siemens, Erlangen, Germany; Abacus Junior Vet, Diatron, Wien, Austria) and serum chemistry (Cobas 6000, Roche, Mannheim, Germany) were collected in potassium‐EDTA and plain tubes with gel separators, respectively, and analyzed within 60 minutes from collection. Urinalysis, including dipstick analysis, measurement of specific gravity by refractometry, sediment cytology (automated [SediVue Dx, IDEXX Laboratories, Westbrook, ME] or by manual microscopy), and bacterial culture and sensitivity, was done on urine samples obtained by cystocentesis. Hematuria and pyuria were defined as the presence of >5 erythrocytes or leukocytes (respectively) in a high power field. Bacteriuria was considered positive if bacteria were evident upon sediment cytology. Proteinuria was defined as a urine dipstick result of ≥1+ (ie, >30 mg/dL).
3
Comprehensive Diagnostic Workup for Canine AKI
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Blood samples were collected in potassium‐EDTA tubes for CBC (Advia 120 or 2120, Siemens, Erlangen, Germany; Abacus Junior Vet, Diatron, Wien, Austria), and whole blood samples in plain tubes with gel separators for serum biochemistry (Cobas 6000, Roche, Mannheim, Germany). Samples obtained in‐house were analyzed within 60 minutes after collection. The CBC and serum biochemistry results from referring clinics were considered if obtained ≤24 hours before admission or during the follow‐up period. Cystocentesis was used to collect urine samples for dipstick chemistry (Urilux, Roche, Mannheim Germany), measurement of specific gravity by refractometry, sediment evaluation, which was done either by experienced laboratory personal or automatically (SediVue Dx, IDEXX Laboratories, Westbrook, ME), and bacterial culture. Pyuria and hematuria were defined as presence of >5 WBCs or RBCs, respectively, per high‐power field (hpf). Proteinuria was defined as a urine dipstick result of ≥1+ (ie, ≥30 mg/dL). The UPC (Cobas Integra 400 Plus or Cobas 6000, Roche, Mannheim, Germany) only was measured in dogs with severe proteinuria (urine dipstick result +4), inactive sediment, and when glomerular disease was suspected as the inciting cause for AKI.
4
Urine Microscopy and SARS-CoV-2 Detection
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Urine microscopy scoring was completed in a subset of 59 patients at the Johns Hopkins site, using the IDEXX SediVue Dx platform,23 (link) with all 59 urine microscopy samples generating an automated report with 70 images each, which were reviewed manually. All urine microscopy measurements were made on the same urine samples for which biomarkers were measured in patients at the Johns Hopkins Hospital. The urine microscopy score was determined based on the number of renal tubular epithelial cells per high-power field, and number of granular casts per low-power field.24 (link) We used reverse transcriptase–polymerase chain reaction (RT-PCR) testing to evaluate for the presence of the SARS-CoV-2 virus in urine samples of 55 patients from the Johns Hopkins Hospital.
5
Canine Acute Kidney Injury Diagnostic Workup
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Blood samples for CBC (Advia 120 or 2120, Siemens, Erlangen, Germany; Abacus Junior Vet, Diatron, Wien, Austria) and serum chemistry (Cobas 6000, Roche, Mannheim, Germany) were collected at presentation in potassium‐EDTA and plain tubes with gel separators, respectively, and analyzed within 60 minutes from collection. CBC and serum chemistry from referring clinics were considered only if obtained ≤24 hours before admission. Urine samples were obtained within 24 hours of presentation by cystocentesis for urinalysis, including dipstick chemistry (Urilux, Roche, Mannheim Germany), measurement of specific gravity by refractometry, sediment evaluation, which was done either by experienced laboratory personnel or automatically (SediVue Dx, IDEXX Laboratories, Westbrook, ME), and aerobic bacterial culture and sensitivity. Pyuria and hematuria were defined as the presence of >5 leukocytes or erythrocytes, respectively in a high‐power field. Proteinuria was defined as a urine dipstick result of ≥1+ (ie, >30 mg/dL). UPC (Cobas Integra 400 Plus or Cobas 6000, Roche, Mannheim, Germany) was only measured in dogs with severe proteinuria (urine dipstick result +4), inactive sediment, and when glomerular disease was suspected as the inciting cause for AKI.
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