Whole cell lysates were prepared in NP-40 buffer containing protease inhibitor cocktail (ThermoFisher Scientific). Immunoblotting was performed with anti-MUC1-C (62 (link)), anti-RASSF1A (Abcam, Cambridge, MA, USA), anti-β-actin (Sigma), anti-ZEB1, anti-DNMT3b, anti-pMEK(S217/S221), anti-MEK, anti-pERK(T202/Y204) and anti-ERK (Cell Signaling Technologies, Danvers, MA, USA).
Anti pmek s217 s221
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-pMEK(S217/S221) is a primary antibody that specifically recognizes the phosphorylated forms of MEK1 and MEK2 at serine residues 217 and 221. This antibody can be used for the detection and analysis of activated MEK proteins in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (2 mentions)
Anti erk, by Cell Signaling Technology (2 mentions)
Anti zeb1, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions)
Anti rassf1a, by Abcam (2 mentions)
Anti p erk t202 y204, by Cell Signaling Technology (2 mentions)
Anti dnmt3b, by Cell Signaling Technology (2 mentions)
Anti mek, by Cell Signaling Technology (2 mentions)
2 protocols using anti pmek s217 s221
1
Protein Expression Analysis of Cell Lysates
2
Protein Expression Analysis of Cell Lysates
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Whole cell lysates were prepared in NP-40 buffer containing protease inhibitor cocktail (ThermoFisher Scientific). Immunoblotting was performed with anti-MUC1-C (62 (link)), anti-RASSF1A (Abcam, Cambridge, MA, USA), anti-β-actin (Sigma), anti-ZEB1, anti-DNMT3b, anti-pMEK(S217/S221), anti-MEK, anti-pERK(T202/Y204) and anti-ERK (Cell Signaling Technologies, Danvers, MA, USA).
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