Tri polyphosphate

High molecular weight chitosan (Mw: 140–190 kDa, degree of deacetylation: > 85%, the viscosity: 200–400 mPa.s, cat. no. C105802) was purchased from Aladdin, Shanghai, China. N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC), N-Hydroxysuccinimide (NHS) and Tri-polyphosphate (TPP) were purchased from Macklin, China; Piperacillin, Ceftazidime and Amikacin were purchased from Sigma-Aldrich, St. Louis, MO, USA. All other chemicals used in this study were of analytical grade.

Lysozyme were purchased from Solarbio (Beijing, China). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC), N-Hydroxysuccinimide (NHS) and Tri-polyphosphate (TPP) were purchased from Macklin (Shanghai, China). Chitosan (DD: 93%; viscosity: 20 mPa·s) were obtained from Aladdin (Shanghai, China). P. aeruginosa, K. pneumoniae, S. aureus, and E. coli were obtained from the Affiliated Hospital of Qingdao University (AHQU, Qingdao, China) and kept in frozen glycerol stock (maintained at −80 °C). Bacteria were cultured overnight in LB broth (Mdbio, Inc, Qingdao, China) at 37 °C for further assays. All other reagents used were analytical grade.