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Pcb 126

Manufactured by Cambridge Isotopes
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PCB 126 is a laboratory reference standard for polychlorinated biphenyl (PCB) congener analysis. It is a pure chemical compound used for the calibration and quantification of PCB congeners in environmental and biological samples.

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Lab products found in correlation

Bile acid standards, by Merck Group (1 mentions) Deuterated internal standards, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sybr green, by Merck Group (1 mentions) N hexane, by Merck Group (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) H2so4, by Merck Group (1 mentions) Na2so4, by Merck Group (1 mentions) Nahco3, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by LGC (1 mentions) Mgso4 7h2o, by Merck Group (1 mentions) Cacl2 2h2o, by Merck Group (1 mentions) 3 4 dichloroaniline, by Merck Group (1 mentions) Tricaine methanesulfonate ms 222, by Merck Group (1 mentions)

3 protocols using pcb 126

1

Isotopic Labeling for Bile Acid Analysis

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Sodium 3-(trimethylsilyl) [2,2,3,3-2H4] propionate (TSP), D2O (99.9% in D), and PCB 126 were ordered from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). Bile acid standards and deuterated internal standards were obtained from Sigma-Aldrich (St Louis, MO, USA) and Cayman Chemical (Ann Arbor, MI, USA).
Tian Y., Rimal B., Gui W., Koo I., Smith P.B., Yokoyama S, & Patterson A.D. (2022). Early Life Polychlorinated Biphenyl 126 Exposure Disrupts Gut Microbiota and Metabolic Homeostasis in Mice Fed with High-Fat Diet in Adulthood. Metabolites, 12(10), 894.
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2

POPs and Fluorescent Dye Protocol

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POPs including TCDD, TCDF, PCB-126, and PCB-153 (Figure 1(c)) were purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA) and suspended in dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MO). The fluorescent dyes including SybrGreen, propidium iodide (Pi), carboxyfluorescein diacetate (CFDA), and bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4) were ordered from Sigma-Aldrich (St. Louis, MO) and Invitrogen (Carlsbad, CA).
Tian Y., Gui W., Rimal B., Koo I., Smith P.B., Nichols R.G., Cai J., Liu Q, & Patterson A.D. (2020). Metabolic impact of persistent organic pollutants on gut microbiota. Gut Microbes, 12(1), 1848209.
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3

Analytical Standards Preparation Protocol

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Ehrenstorfer GmbH. Dimethyl sulfoxide, 3,4-dichloroaniline, Tricaine methane sulfonate (MS222), n-hexane, CaCl2.2H2O, MgSO4.7H2O, NaHCO3, KCl, Na2SO4 and H2SO4 were obtained from Sigma-Aldrich Co. 13 C12 isotope-labelled PCB126 was obtained from Cambridge Isotope Laboratories (CIL) and was part of a prepared mixture of 12 13 C12 isotope labelled PCBs (CIL EC 4937). 13 C12 labelled PCB70 was from the same source (CIL EC 4914).
Di Paolo C., Groh K.J., Zennegg M., Vermeirssen E.L., Murk A.J., Eggen R.I., Hollert H., Werner I, & Schirmer K. (2015). Early life exposure to PCB126 results in delayed mortality and growth impairment in the zebrafish larvae. Aquatic toxicology (Amsterdam, Netherlands), 169.
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