Standard compounds such as quercetin, quercetin-3,4′-diglucoside and quercetin-4′-glucoside were purchased from Sigma–Aldrich, Korea. The flavonoid standards were dissolved in 80% MeOH at several concentrations and a high linearity was obtained from each curve. quercetin, quercetin-3,4′-diglucoside and quercetin-4′-glucoside were identified based on their retention times and their concentrations were calculated by comparing the peak areas of samples with standards and correlated with obtained results.
Quercetin 4 glucoside
Manufactured by Merck Group
Quercetin-4'-glucoside is a flavonoid compound found in various plant sources. It is a chemical constituent that can be isolated and used for research purposes. The core function of this compound is to serve as a reference standard or an analytical tool for identification and quantification in chemical analysis and research applications.
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Lab products found in correlation
2 protocols using quercetin 4 glucoside
1
Quantitative Analysis of Quercetin Glycosides
2
Quantification of Quercetin Compounds in Onion and Garlic
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The analysis of the onion and garlic extracts was performed on an Agilent 1100 HPLC system. The column used was a Phenomenex Luna 5u C18 (2) (150 x 4.6 mm, 5um). The mobile phase consisted of (A) 50 mM acetic acid (HAC) in Millipore ultrapure water with 5% acetonitrile (v/v) and (B) acetonitrile with 5% methanol (v/v). The flow rate was 1.0mL min-1 and the injection volume 10 uL. The double gradient used was as follows: 0-2 min, 0% eluent B; 2-17 min, 0-45% B; 17-20 min, 45-80% B; 20-21 min, 80% B; 21-23 min, 80-0% B; 23-35 min, 0% B. External standards used for identification and quantification were quercetin (SigmaAldrich Chemie Gmbh) and quercetin 4-glucoside (Extra synthase). The absorbance will be measured at 370 nm using an Agilent 1100 (G1315B) diode array detector (Agilent Technologies). Results will be presented as milligrams (mg) of quercetin 4 glucoside equivalent per kilogram fresh weight of onion (mg kg -1 fw) for all forms of quercetin.
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