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Anti pcna pc10 2586

Manufactured by Cell Signaling Technology
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Anti-PCNA (PC10; 2586) is a mouse monoclonal antibody that recognizes the proliferating cell nuclear antigen (PCNA) protein. PCNA is a key regulator of DNA replication and repair processes.

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Lab products found in correlation

Sb203580 s1076, by Selleck Chemicals (1 mentions) Mouse anti pd1 be0146, by BioXCell (1 mentions) Il 22, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti pcna pc10 2586

1

Antibody and Inhibitor Procurement Protocol

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Anti-ZNF207 (PA5-53535, PA5-30641, #703747) and anti-CD3z (4B10) (MA5-15608) antibodies were purchased from Invitrogen (Waltham, Massachusetts, USA). Anti-beta actin (ab8226) antibody was purchased from Abcam (Cambridge, UK). Anti-HIF-1α (D1S7W; #36169), anti-HIF-2α (D6T8V; #59973), anti-CD8α (D4W2Z; #98941), and anti-PCNA (PC10; 2586) antibodies were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Mouse C-X3-C-motif ligand (CX3CL1)/fractalkine chemokine domain antibody (MAB571) was purchased from R&D Systems (Minneapolis, Minnesota, USA). Anti-glyceraldehyde 3-phosphatedehydrogenase (GAPDH) (UPS002) was purchased from Upsbiotech. Mouse anti-PD1 (BE0146) and rat IgG2a isotype control antibody (BE0089) were purchased from Bio X Cell (Lebanon, New Hampshire, USA). Bay 87–2243 (S7309), PT2385 (S8352), PF-06840003 (S8657), and SB203580 (S1076) were purchased from Selleck (Houston, Texas, USA).
Wang X., Zhou T., Chen X., Wang Y., Ding Y., Tu H., Gao S., Wang H., Tang X, & Yang Y. (2022). System analysis based on the cancer–immunity cycle identifies ZNF207 as a novel immunotherapy target for hepatocellular carcinoma. Journal for Immunotherapy of Cancer, 10(3), e004414.
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2

Western Blot and Immunofluorescence Assays

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The primary antibodies used for Western blots were anti-EZH2 (D2C9, 5246S, Cell Signaling Technology, Danvers, MA), anti-H3K27me3 (Lys27) (C36B11, 9733, Cell Signaling Technology), anti-total H3 (D1H2, 4499S, Cell Signaling Technology), anti-KLK8 (14232-1-AP, Proteintech, Chicago, Illinois). Antibodies used for Immunofluorescence staining were anti-EZH2 (MA5-15101, Thermo Scientific, Waltham, MA), anti-Ki67 (8D5, 9449, Cell Signaling Technology), anti-PCNA (PC10, 2586, Cell Signaling Technology). GSK126 (SC0060) was purchased from Beyotime biotechnology (Shanghai, China). Recombinant Human IL-17 (IL-17A), IL-22, TNF-α, and IFN-γ were purchased from Peprotech (Rocky Hill, New Jersey).
Zhang T., Yang L., Ke Y., Lei J., Shen S., Shao S., Zhang C., Zhu Z., Dang E, & Wang G. (2020). EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation. Cell Death & Disease, 11(10), 826.
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