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Nis element microscope imaging software

Manufactured by National Instruments

NIS-Element Microscope Imaging software is a comprehensive imaging and analysis platform designed for researchers and scientists working with microscopes. The software provides advanced tools for image acquisition, processing, analysis, and management, enabling users to capture, visualize, and quantify data from their microscopic experiments.

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2 protocols using nis element microscope imaging software

1

Quantifying Cell Monolayer Anisotropy

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Phase-contrast images of cell monolayers were taken at X10 magnification with a Nikon TE200 microscope with a Nikon DS-Fi1c camera and DS-U2 PC control unit and the NIS-Element Microscope Imaging software. Pseudo-coloured images were obtained with the OrientationJ plugin55 (link),56 (link) of ImageJ using a Gaussian window of 2 pixels, a Fourier gradient, and colours representing orientation (hue) and coherency (saturation). Fibre anisotropy was calculated using the Fibriltool macro57 (link) by analysing 5 square (200 × 200 pixels) regions of interest per image and 4 images per condition in each experiment.
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2

Characterizing Alginate-Encapsulated HepG2 Cells

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Phase contrast images of alginate HepG2 cell beads were taken with a Nikon TE200 microscope equipped with a Nikon DS-Fi1c camera and DS-U2 PC control unit. The NIS-Element Microscope Imaging software was used to measure the diameter and aspect ratio of the imaged beads.
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