Sections (5 µm) were cut for immune staining. The tissue sections were incubated with primary antibodies of IL-1 β, TNF- α, NF-κβ, and VEGF (anti-IL-1β (SC-12742), anti-TNF-α (SC-52746), anti-NF-κβ (SC-8008), and anti-VEGF (SC-57496), Santa Cruz, Biotechnology, Inc., at a dilution of 1:200) for 1 h at room temperature, followed by washing with TBS. Endogenous peroxidases were blocked using hydrogen peroxide. Afterward, an HRP-labeled detection kit (Bio SB, BSB0001, USA) was used as per the manufacturer’s instructions to develop the positive reaction. Negative controls were obtained by deleting incubation with primary antibodies. Positive immune staining was quantified as area percent using LAS-X software (Leica v4.13, Germany).
Anti il 1β sc 12742
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-IL-1β (SC-12742) is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect the Interleukin-1 beta (IL-1β) protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti tnf α sc 52746, by Santa Cruz Biotechnology (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Las x software, by Leica (1 mentions)
Anti nf κβ sc 8008, by Santa Cruz Biotechnology (1 mentions)
Chemiluminescent imaging system, by Syngene (1 mentions)
Anti asc, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Sc 514414, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
High performance liquid chromatography (hplc), by Agilent Technologies (1 mentions)
Ldh assay kit, by Beyotime (1 mentions)
Rpmi 1640 medium, by Solarbio (1 mentions)
Discovery v8 stereo microscopes, by Zeiss (1 mentions)
Axio scope a1, by Zeiss (1 mentions)
3 protocols using anti il 1β sc 12742
1
Quantitative Immunohistochemical Analysis
2
Neuroprotective Effects of Compounds
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The following materials were used: RPMI 1640 medium (Solarbio Life Sciences, Beijing, China), PC12 cell (Mlbio, ml052695, Shanghai, China), SH-SY5Y cells (Mlbio, ml052084, Shanghai, China), MPP+ (Sigma-Aldrich, MO, USA), microplate reader (Thermo Fisher Scientific, MA, USA), LDH assay kit (Beyotime, Shanghai, China), ELISA kits (R&D Systems, Minneapolis, USA), RIPA total protein lysate (Abcam, MA, USA), PVDF membranes (Thermo Fisher Scientific, USA), anti-TH (sc-25269, Santa Cruz Biotechnology, CA, USA), anti-NLRP3 (SAB1410191, Sigma-Aldrich, USA), anti-caspase-1 (PRS3459, Sigma-Aldrich, USA), anti-ASC (sc-514414, Santa Cruz Biotechnology, USA), anti-IL-1β (sc-12742, Santa Cruz Biotechnology, USA), anti-IL-18 (PA5-79481, Thermo Fisher Scientific, USA), and anti-GAPDH (G9545, Sigma-Aldrich, USA), HRP-conjugated secondary antibody (sc-2357, sc-2789, Santa Cruz Biotechnology, USA), chemiluminescent imaging system (Syngene, Cambridge, UK), HPLC (Agilent Technologies, CA, USA).
3
Histological and Immunohistochemical Analysis of Brain Tissue
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Brains fixed in 4% paraformaldehyde were processed for paraffin embedding. Hematoxylin and eosin (H&E) staining was conducted on 5 μm sections according to previously published procedures (Wang et al., 2013 (link)). Immunohistochemistry (IHC) analyses were performed on hippocampus sections with the following antibodies: Anti-IL-1β (sc-12742, 1:200, Santa Cruz), Anti-TNF-α (sc-52746, 1:200, Santa Cruz), Anti-Synaptophysin (MAB5258-I, 1:200, Millipore). Zeiss Axio Scope.A1 or Zeiss Discovery.V8 Stereo microscopes (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) was used and integrated with an Axio-Cam ICc3 camera (Spectra Service, Ontario, NY, United States). Images were acquired by AxioVision Rel. 4.7 software from Zeiss. The images were digitized and quantified using ImageJ.
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