CD1 wild-type mice were injected intraperitoneally with a neutrophil depletion kit InVivoPlus anti-mouse Ly6G (Bio X Cell), diluted in InVivoPlus pH 7.0 Dilution Buffer (Bio X Cell). The animals were injected with an initial dose of 400 μg of antibody 2 h before surgery, then after every 3 days 100 μg of antibody was injected. The animals were collected on the first day after damage and 28 days after damage (n = 3 per treatment and timepoint). In total the animals collected at the 28 days after damage received ten injections A total of 12 animals were used for this experiment.
Invivoplus anti mouse ly6g
Manufactured by BioXCell
Sourced in Canada, United States
InVivoPlus anti-mouse Ly6G is a laboratory product used for the detection and analysis of Ly6G, a cell surface marker expressed on mouse neutrophils. It can be used in various research applications involving the identification and characterization of neutrophils in mouse models.
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4 protocols using invivoplus anti mouse ly6g
1
Neutrophil Depletion in CD1 Mice
2
Ly6G Depletion for Immune Induction
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Mice in the Ly6G depletion treatment group were given doses of 250 ug of InVivoPlus anti- mouse Ly6G (Bio X Cell, Burlington, Canada) in 200 uL PBS via intraperitoneal injection the day before DC immunization, the day of immunization, and every 2–3 days throughout the induction phase of the immune response.
3
Ly6G+ Cell Depletion in Muscle Injury
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Five mice were intraperitoneally administered with either 200 μg of Ly6G-specific monoclonal antibody to deplete Ly6G+ cells or IgG2a isotype control antibodies (InVivoPlus anti-mouse Ly6G, Bioxcell, St Louis, NH, USA) 5, 8, 11, 13 days after CS induction. One mouse on each side was cheek bled one day after the first Ly6G-specific monoclonal antibody induction and on the day of scarifice at day 14 to confirm the depletion of Ly6G+ cells via flow cytometry. On day 14, after CS induction, we compared the percentage of neutrophils in right tibialis anterior muscle, grip strength, and the cross-sectional area of left tibialis anterior muscle.
4
Quantifying Sdc1 Response to Neutrophil Depletion
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WT mice were injected 100 µL intraperitoneally with InVivoPlus antimouse Ly6G (2.5 μg/µL, Bio X cell number BP0075; Major Resources Table in the online-only Data Supplement) or saline. 21 (link) Twenty-four hours later the mice underwent intra-arterial administration of 150 µL of human MPO, Q91T, polylysine, or HSA (all 0.1 μg/µL). The mice were treated for 15 minutes, after which blood from the vena cava was collected. The plasma was further used for quantification of Sdc1 using mouse Sdc1 ELISA (Cloud-Clone Corp number SEB966Mu).
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