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3 nitrotyrosine antibody

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3'-Nitrotyrosine antibody is a laboratory reagent used for the detection and quantification of 3'-nitrotyrosine, a post-translational modification that serves as a marker of nitrosative stress. This antibody can be used in various immunoassays and analytical techniques to study the presence and levels of 3'-nitrotyrosine in biological samples.

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Lab products found in correlation

Rneasy kit, by Qiagen (2 mentions) Iec 6, by American Type Culture Collection (2 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (2 mentions) Nf kbp65 f 6 antibody, by Santa Cruz Biotechnology (2 mentions) Ncbi primer blast online, by Integrated DNA Technologies (2 mentions) Quantitect reverse transcription, by Qiagen (2 mentions)

Close spelling variants of this product

3-nitrotyrosine (13 citations) Nitrotyrosine (7 citations) Anti-3-Nitrotyrosine antibody (4 citations)

2 protocols using 3 nitrotyrosine antibody

1

Intestinal Epithelial Cell TUNEL and Nitrotyrosine Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNeasy® kit (catalogue no. 74106; Qiagen) and QuantiTect® Reverse Transcription (catalogue no. 205313; Qiagen), TUNEL kit (In Situ Cell Death Detection Kit, Fluorescein, Catalogue no. 11684795910; Roche), 3’-Nitrotyrosine antibody (Catalogue no. ab61392; Abcam), NF-kBp65(F-6) antibody (catalogue no. sc-8008; Santa Cruz Biotechnology), 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, catalogue no. D9542; Sigma), LPS, Lipopolysaccharide from Escherichia coli 0127:B8 (Sigma catalogue no. L3129). Forward and reverse primers were custom designed using NCBI Primer-BLAST online program and ordered from Integrated DNA Technologies, and appear in Table. 1 The small intestinal epithelial cell IEC-6 was obtained from American Type Culture Collection (ATCC, Manassas, VA). TUNEL was from Roche Life sciences. Quantitative RT-PCR was performed using primers in Table. 1 as described(15 ). All human intestine was obtained via a waiver of consent from the Office of Human Subjects Research, Johns Hopkins University (IRB00094036) and was collected in a deidentified manner. GOS (galacto-oligosaccharides) was from Biosynth Carbosynth (Newbury, Berkshire, UK).
Sodhi C.P., Wipf P., Yamaguchi Y., Fulton W.B., Kovler M., Niño D.F., Zhou Q., Banfield E., Werts A.D., Ladd M.R., Buck R.H., Goehring K.C., Prindle T J.r., Wang S., Jia H., Lu P, & Hackam D.J. (2020). The human milk oligosaccharides 2’-Fucosyllactose and 6’-Sialyllactose protect against the development of necrotizing enterocolitis by inhibiting Toll-Like Receptor 4 signaling.. Pediatric research.
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2

Intestinal Epithelial Cell TUNEL and Nitrotyrosine Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNeasy® kit (catalogue no. 74106; Qiagen) and QuantiTect® Reverse Transcription (catalogue no. 205313; Qiagen), TUNEL kit (In Situ Cell Death Detection Kit, Fluorescein, Catalogue no. 11684795910; Roche), 3’-Nitrotyrosine antibody (Catalogue no. ab61392; Abcam), NF-kBp65(F-6) antibody (catalogue no. sc-8008; Santa Cruz Biotechnology), 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, catalogue no. D9542; Sigma), LPS, Lipopolysaccharide from Escherichia coli 0127:B8 (Sigma catalogue no. L3129). Forward and reverse primers were custom designed using NCBI Primer-BLAST online program and ordered from Integrated DNA Technologies, and appear in Table. 1 The small intestinal epithelial cell IEC-6 was obtained from American Type Culture Collection (ATCC, Manassas, VA). TUNEL was from Roche Life sciences. Quantitative RT-PCR was performed using primers in Table. 1 as described(15 ). All human intestine was obtained via a waiver of consent from the Office of Human Subjects Research, Johns Hopkins University (IRB00094036) and was collected in a deidentified manner. GOS (galacto-oligosaccharides) was from Biosynth Carbosynth (Newbury, Berkshire, UK).
Sodhi C.P., Wipf P., Yamaguchi Y., Fulton W.B., Kovler M., Niño D.F., Zhou Q., Banfield E., Werts A.D., Ladd M.R., Buck R.H., Goehring K.C., Prindle T J.r., Wang S., Jia H., Lu P, & Hackam D.J. (2020). The human milk oligosaccharides 2’-Fucosyllactose and 6’-Sialyllactose protect against the development of necrotizing enterocolitis by inhibiting Toll-Like Receptor 4 signaling.. Pediatric research.
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