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Acetylcholine ach

Manufactured by Fujifilm
Sourced in United States, Japan

Acetylcholine (ACh) is a neurotransmitter that plays a fundamental role in the function of the nervous system. It is responsible for the transmission of signals between neurons and target cells, including muscles, glands, and certain types of neurons. ACh is a key component in the regulation of various physiological processes, including muscle contraction, autonomic nervous system function, and cognitive processes.

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2 protocols using acetylcholine ach

1

Endothelial Function Measurement Protocol

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Endothelial function was measured using isometric tension, as previously reported (n = 11).22, 23, 24 Briefly, the CC strips were equilibrated for a minimum of 60 min in an aerated organ bath containing Kreb's solution (119 mM NaCl, 4.6 mM KCl, 1.5 mM CaCl2, 1.2 mM MgCl2, 15 mM NaHCO3, 11 mM d‐glucose, and 1.2 mM NaH2PO4) at 37°C with 5% CO2. The resting force for tissues was set to 500 mg, and changes in isometric tension were recorded using a force transducer (Nihon Kohden) connected to a data acquisition board (PowerLab 4/26). Relaxant experiments were conducted using strips pretreated with 10 μM noradrenaline (NA; Sigma Aldrich, MO, USA); the relaxant effect was induced by acetylcholine (ACh; Wako Pure Chemical Industries) and sodium nitroprusside (SNP; Sigma Aldrich). Cumulative dose (10–10 to 10–4 M) response curves were obtained for ACh and SNP using different tissue specimens.
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2

Organophosphate Degradation Protocols

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Chemical standards of diazinon and diazinon-oxon (Fig. S1), S9, cofactors (glucose-6-phosphate, β-NADPH, and β-NADH), acetylcholine (ACh), and Ch were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). AChE derived from human erythrocytes was purchased from Merck KGaA (Darmstadt, Germany). Sodium hypochlorite (guaranteed reagent grade), which is used in full-scale water treatment plants, was purchased from AGC Inc. (Tokyo, Japan). All chemicals were used without any further purification.
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