TALEN proteins, all containing a 3xFLAG tag, were expressed by in vitro transcription/translation. 800 ng of TALEN-encoding plasmid or no plasmid (“empty lysate” control) was added to an in vitro transcription/translation reaction using the TNT® Quick Coupled Transcription/Translation System, T7 Variant (Promega) in a final volume of 20 μL at 30 °C for 1.5 h. Western blots were used to visualize protein using the anti-FLAG M2 monoclonal antibody (Sigma-Aldrich). TALEN concentrations were calculated by comparison to standard curve of 1 ng to 16 ng N-terminally FLAG-tagged bacterial alkaline phosphatase (Sigma-Aldrich).
N terminally flag tagged bacterial alkaline phosphatase
Manufactured by Merck Group
N-terminally FLAG-tagged bacterial alkaline phosphatase is a recombinant enzyme produced in bacteria that has a FLAG peptide sequence attached to the N-terminus. Alkaline phosphatase is an enzyme that catalyzes the hydrolysis of phosphate groups from various molecules.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n terminally flag tagged bacterial alkaline phosphatase
1
In Vitro TALEN Protein Expression
2
In Vitro TALEN Protein Expression
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