Before electroporation, Jurkat cells were harvested by centrifugation (10 min, 200× g, 4 °C) and resuspended in an appropriate electroporation medium. The cell concentration was determined using a Coulter counter (Z2, Beckman Coulter, Brea, CA, USA). The cell suspension (3.0 μL) containing 1.0 × 105 Jurkat cells was dispensed in the silicone oil, and a high voltage was supplied using a DC high-voltage (HV) power supply (HAR-30R10, Matsusada Precision, Kusatsu, Japan). When the applied voltage was set to 3.0 kV, instantaneous short-circuiting could be induced. After short-circuiting, with a distinctive sound, the DC HV power supply was manually turned off.
Kf 96 100cs
KF-96-100CS is a silicone fluid product manufactured by Shin-Etsu Chemical. It is a clear, colorless liquid with a viscosity of 100 centistokes. The product is designed for use as a lubricant, damping fluid, and heat transfer medium in various industrial applications.
Lab products found in correlation
6 protocols using kf 96 100cs
Electroporation of Jurkat Cells in Silicone Oil
Before electroporation, Jurkat cells were harvested by centrifugation (10 min, 200× g, 4 °C) and resuspended in an appropriate electroporation medium. The cell concentration was determined using a Coulter counter (Z2, Beckman Coulter, Brea, CA, USA). The cell suspension (3.0 μL) containing 1.0 × 105 Jurkat cells was dispensed in the silicone oil, and a high voltage was supplied using a DC high-voltage (HV) power supply (HAR-30R10, Matsusada Precision, Kusatsu, Japan). When the applied voltage was set to 3.0 kV, instantaneous short-circuiting could be induced. After short-circuiting, with a distinctive sound, the DC HV power supply was manually turned off.
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